Accéder au contenu
Merck

Principaux documents

Voir toute la documentation

SAE0094

SAvPhire™ Monomeric Streptavidin

High-affinity biotin binding—reduces aggregation and enables precise, reliable detection and purification of biotinylated molecules

Synonyme(s) :

mSA3

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue

A propos de cet article

UNSPSC Code:
12352202
NACRES:
NA.25
Form:
lyophilized powder
Recombinant:
expressed in E. coli
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

SAvPhire™ Monomeric Streptavidin, recombinant, expressed in E. coli, High Affinity

recombinant

expressed in E. coli

form

lyophilized powder

shipped in

wet ice

storage temp.

−20°C

General description

Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag

Biochem/physiol Actions

SAvPhire™ Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire™ Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.
The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include:
  • Minimizing distribution or dynamics in the probing of biotinylated targets
  • Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules
  • Easier access to desired target

This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.

Preparation Note

Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.
This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows:

  • 1 mg vial: add 250 μL of water for a 4 mg/mL solution
  • 5 mg vial: add 1.25 mL of water for a 4 mg/mL solution

The reconstituted product can be further diluted in water or PBS as needed.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SAvPhire is a trademark of Sigma-Aldrich Co. LLC

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

Si vous avez besoin d'assistance, veuillez contacter Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents