SAB4200658

Monoclonal Anti-EPS8 antibody produced in mouse

clone EPS8-2, purified from hybridoma cell culture

• Synonyme(s) : DFNB102, epidermal growth factor receptor pathway substrate 8
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: EPS8-2
• Forme: buffered aqueous solution
• Poids mol.: antigen ~90 kDa
• Espèces réactives: human
• Concentration: ~1 mg/mL
• Technique(s): flow cytometry: 10-20 μg/test using using A549 cells.; immunoblotting: 2.5-5 μg/mL using using whole extracts of HepG2 cells.; immunofluorescence: 10-20 μg/mL using using whole extracts of A549 cells.; immunoprecipitation (IP): suitable
• Isotype: IgG2a
• Numéro d'accès UniProt: Q12929
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... EPS8(2059)

Description

Description générale

Monoclonal Anti- EPS8 (mouse IgG2a isotype) is derived from the hybridoma EPS8-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8 (GeneID: 2059), conjugated to Key loop hemocyanin (KLH). Epidermal growth factor receptor kinase substrate 8 (EPS8) is also known as DFNB102 (autosomal recessive deafness-102). EPS8 is also found to be an actin filament barbed end capping protein.

Immunogène

synthetic peptide corresponding to a sequence at the N-terminal region of human EPS8

Application

Monoclonal Anti-EPS8 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence
• flow cytometry

Actions biochimiques/physiologiques

Epidermal growth factor receptor kinase substrate 8 (EPS8) serves as a substrate for the kinase activity of epidermal growth factor receptor (EGFR). It is essential for cell motility and regulation of actin filaments growth. EPS8 scaffolds together with Abl interactor 1 (Abi1) and SOS Ras/Rac guanine nucleotide exchange factor 1 (Sos1) to a trimeric complex which is required for activation of the small guanosine triphosphatase (GTPase) Rac. On the other hand, EPS8 form complexes with related to the N terminus of tre oncogene (RN-tre), a Rab5 GTPase activating protein, to inhibit EGFR internalization. EPS8 is involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration, cancer cell migration and invasion. It is reported to be expressed at elevated levels in a range of human malignancies. This protein is also involved in spine morphogenesis and plasticity and may characterize forms of intellectual disabilities. For instance, reduced EPS8 levels were reported in brains of autism patients.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable