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SAB4200534

Sigma-Aldrich

Anti-Claudin-1 (C-terminal) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-Claudin-1 (C-Terminal), antibody produced in rabbit, affinity isolated antibody

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~23 kDa

Espèces réactives

human, dog

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

~1.0 mg/mL

Technique(s)

immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human colon.
indirect immunofluorescence: 1.0-2.0 μg/mL using MDCK cells.
western blot: 0.5-1.0 μg/mL using extracts of HEK-293T cells overexpressing human claudin-1.

Numéro d'accès UniProt

O95832

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CLDN1(9076)

Description générale

Claudin-1, also known as claudin 1 (CLDN1), seminal metalloprotease-1 (Semp1) and ichthyosis, leukocyte vacuoles, alopecia, and sclerosing cholangitis (ILVASC), is expressed at high levels in kidney and liver and at lower levels in spleen, heart, brain, lung and testis. Claudins are characterized with four transmembrane domains and two extracellular loops, required to form tight junction strands.

Immunogène

synthetic peptide corresponding to a sequence at the C-terminus of human claudin-1, conjugated to KLH. The corresponding sequence is highly conserved in mouse claudin-1 (single amino acid substitution) and in rat claudin-1 (90% identity).

Application

Anti-Claudin-1 (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Actions biochimiques/physiologiques

Claudin-1 directly interacts with tight junction (TJ) -associated peripheral membrane proteins zonula occludens (ZO) proteins -1/2/3. Defects in the gene encoding claudin-1 are the cause of an autosomal recessive syndrome named ichthyosis-sclerosing cholangitis neonatal (NISCH). Claudins′ expression is altered in several human cancers. Claudin-1 is frequently up-regulated in colorectal carcinomas (CRCs), resulting in tumor differentiation and progression.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Claudin-1 involved in neonatal ichthyosis sclerosing cholangitis syndrome regulates hepatic paracellular permeability
Yuan P, et al.
Cell research, 25, 157-168 (2014)
Transmembrane Proteins in the Tight Junction Barrier
Fanning A S, et al.
Journal of the American Society of Nephrology, 10, 1337-1345 (1999)
Nga Le et al.
Cellular and molecular gastroenterology and hepatology, 11(1), 55-76 (2020-07-14)
Communication between T cells and the intestinal epithelium is altered in many diseases, causing T-cell activation, depletion, or recruitment, and disruption of the epithelium. We hypothesize that activation of T cells regulates epithelial barrier function by targeting the assembly of
Pengfei Yuan et al.
Cell research, 25(2), 157-168 (2014-12-31)
As a gram-positive, spore-forming anaerobic bacillus, Clostridium difficile (C. difficile) is responsible for severe and fatal pseudomembranous colitis, and poses the most urgent antibiotic resistance threat worldwide. Epidemic C. difficile is the leading cause of antibiotic-associated diarrhoea globally, especially diarrhoea
Angélica Cruz-Lebrón et al.
Gut microbes, 13(1), 1946368-1946368 (2021-07-28)
Over the past three decades the United States has experienced a devastating opioid epidemic. One of the many debilitating side effects of chronic opioid use is opioid-induced bowel dysfunction. We investigated the impact of methadone maintenance treatment (MMT) on the

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