S7897

Sarcosine Oxidase from Bacillus sp.

lyophilized powder, 25-50 units/mg solid

• Synonyme(s) : Sarcosine: oxygen oxidoreductase (demethylating)
• Numéro CAS: 9029-22-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-850-5
• MDL number: MFCD00132221
• Numéro CE : 1.5.3.1 (BRENDA, IUBMB)
• Specific activity: 25-50 units/mg solid

Propriétés

• form: lyophilized powder
• Quality Segment: 200
• specific activity: 25-50 units/mg solid
• mol wt: 44 kDa
• storage temp.: −20°C

Description

General description

Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline.

Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD.

Application

Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV. It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor.

Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins.

Physical form

No stabilizers added

Other Notes

One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mot-clé: Danger
• Codes de danger: H334
• Mentions de précaution: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, type N95 (US)