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Key Documents

S6022

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, medium

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.52

Qualité

DNA grade
for molecular biology

Gamme de produits

BioReagent

Forme

beads

Gonflement

1 g swells to 9-11 mL

Diamètre des billes

50-150 μm

Dimension de pores

exclusion limit (20 bp dsDNA; (rA)20)

pH

2-10

Activité étrangère

DNase, RNase, none detected

Température de stockage

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

Clé InChI

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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Description générale

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.

Caractéristiques et avantages

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Informations légales

Sephadex is a registered trademark of Cytiva

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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