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S1638

Millipore

Streptavidin−Agarose from Streptomyces avidinii

buffered aqueous suspension

Synonyme(s) :

streptavidin agarose beads, streptavidin agarose resin

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About This Item

Numéro MDL:
MFCD00082035
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Forme

buffered aqueous suspension

Niveau de qualité

200

Ampleur du marquage

≥1 mg per mL

Technique(s)

affinity chromatography: suitable

Matrice

4% beaded agarose

Activation de la matrice

cyanogen bromide

Fixation de matrice

amino

Espaceur de matrice

7 atoms

Capacité

≥15 μg/mL binding capacity (biotin)

Température de stockage

2-8°C

Catégories apparentées

Application

Streptavidin−agarose from Streptomyces avidinii has been used:
  • to pull down biotinylated cell surface proteins during the quantification of plasma membrane transforming growth factor β (TGFβ) receptor II (TβRII) and Tβ
  • RII internalization
  • in biotinylated miRNA pull-down assay; as secondary antibodies in immunoprecipitation

Streptavidin-agarose is used in protein chromatography, affinity chromatography, and recombinant protein expression and analysis. Streptavidin-agarose has been used to study the oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins. Streptavidin-agarose has also been used to develop a method for screening triplex DNA binders from natural plant extracts.
Used for the purification of biotin containing proteins or DNA binding proteins

Actions biochimiques/physiologiques

Streptavidin is a homotetrameric protein, isolated from Streptomyces avidinii, which, like avidin, has a high affinity for biotin. Streptavidin is slightly anionic (pI ~ 5-6) and non-glycosylated. These properties contribute to its relatively low non-specific binding compared to egg white avidin. Streptavidin is also more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin-agarose can be used to immobilize or isolate various biotinylated macromolecules and complexes (proteins, antibodies, lectins, nucleic acids, receptors, and ligands). The inherent high-affinity streptavidin-biotin interaction requires harsh conditions to release biotinylated macromolecules. This feature makes streptavidin-agarose useful in a variety of affinity purification applications.

Forme physique

Suspension in 0.01 M sodium phosphate, pH 7.2, containing 0.05 M NaCl and 0.02% sodium azide

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000353157
0000353125
0000353157
0000353125
0000310638

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Behrad Derakhshan et al.
Nature protocols, 2(7), 1685-1691 (2007-07-21)
Covalent addition of nitric oxide (NO) to Cys-sulfur in proteins, or S-nitrosylation, plays pervasive roles in the physiological and pathophysiological modulation of mammalian protein functions. Knowledge of the specific protein Cys residues that undergo NO addition in different biological settings
I Gottschalk et al.
European journal of biochemistry, 267(23), 6875-6882 (2000-11-18)
Two cytochalasin B-binding states of the human red blood cell facilitative glucose transporter GLUT1 were studied, one exhibiting one cytochalasin B-binding site on every second GLUT1 monomer (state 1) and the other showing one site per monomer (state 2). Quantitative
Niusheng Xu et al.
Analytical chemistry, 84(5), 2562-2568 (2012-01-10)
A novel ligand fishing assay was established to screen triplex DNA binders from complicated samples by a combination of immobilization of triplex DNA on agarose beads and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The biotinylated oligodeoxynucleotides were first bound to
Veronica Ramberg et al.
Journal of neuroinflammation, 8, 34-34 (2011-04-16)
The transcription factors CCAAT/enhancer binding proteins (C/EBP) α, β and δ have been shown to be expressed in brain and to be involved in regulation of inflammatory genes in concert with nuclear factor κB (NF-κB). In general, C/EBPα is down-regulated
Angel Guerra-Moreno et al.
Science signaling, 12(609) (2019-11-28)
The yeast stress-activated protein kinase Hog1 is best known for its role in mediating the response to osmotic stress, but it is also activated by various mechanistically distinct environmental stressors, including heat shock, endoplasmic reticulum stress, and arsenic. In the
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Contenu apparenté

Technique de pull-down (interactions protéines/protéines)

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Technique de pull-down (interactions protéines/protéines)

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Technique de pull-down (interactions protéines/protéines)

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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Questions

  1. Do the streptavidin beads have disulphide bonds between the dimers?

    1 answer

    1. No analysis of the post-conjugation structure of streptavidin has been performed. This product is tested for binding capacity and functionality. The product S4762 is used in the production of the S1638.

      See the link below for additional information on S4762, streptavidin, including structure details and references:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/896/s4762pis.pdf

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