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RAB0223

Human IFN γ ELISA Kit

Name: Human IFN γ ELISA Kit
Brand: SIGMA

for cell and tissue lysates

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A propos de cet article

NACRES:

NA.77

UNSPSC Code:

41116158

Gene information:

human ... IFNG(3458)

Input:

sample type tissue lysate
sample type cell lysate

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

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Propriétés

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 20.6-15000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IFNG(3458)

Description

General description

The Human IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-γ cell lysate and tissue lysate.

Immunogen

Recombinant Human IFN-γ

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IFNγ ELISA Kit has been used in ELISA assay.

Biochem/physiol Actions

IFNG (interferon-γ) is a type-2 interferon, which is produced by Th (T helper cells)-1 lymphocytes, CD8 cytotoxic lymphocytes, natural killer (NK) cells, and natural killer T (NKT) cells. It plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. High expression of IFNG is common in many autoimmune diseases, such as systemic lupus erythematosis, Sjögren syndrome, inflammatory bowel disease and multiple sclerosis.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Cet article	RAB0227	RAB0284	RAB0183
Sigma-Aldrich RAB0223 Human IFN γ ELISA Kit	Sigma-Aldrich RAB0227 Rat IFN-γ ELISA Kit	Sigma-Aldrich RAB0284 Human IL-1ra ELISA Kit	Sigma-Aldrich RAB0183 Human bFGF ELISA Kit
description for cell and tissue lysates	description for serum, plasma and cell culture supernatant	description for cell and tissue lysates	description for cell and tissue lysates
Gene Information human ... IFNG(3458)	Gene Information rat ... Ifng(25712)	Gene Information human ... IL1RN(3557)	Gene Information human ... FGF2(2247)
species reactivity human	species reactivity rat	species reactivity human	species reactivity human
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 15 pg/mL standard curve range: 20.6-15000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 100 pg/mL standard curve range: 122.9-30000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 100 pg/mL standard curve range: 0.124-30 ng/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 50 pg/mL standard curve range: 102.4-10000 pg/mL
input sample type tissue lysate sample type cell lysate	input sample type cell culture supernatant(s) sample type serum sample type plasma	input sample type cell lysate sample type tissue lysate	input sample type tissue lysate sample type cell lysate
detection method colorimetric	detection method colorimetric	detection method colorimetric	detection method colorimetric
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable

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Description

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ELISA 5X Sample Diluent Buffer (Item D2)
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RABELADE
ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
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RABLYSIS1
2X Cell Lysis Buffer (Item J)
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RABSTOP3
ELISA Stop Solution (Item I)
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ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
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20X Wash Buffer (Item B)
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Pictogrammes

GHS05

Mot-clé

Warning

Codes de danger

H290

Mentions de précaution

P234 - P390

Hazard Classifications

Met. Corr. 1

Classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)