R4526

Reference Dye for Quantitative PCR

100 ×, solution

• Synonyme(s) : Reference dye for qPCR, ROX
• NACRES: NA.55
• UNSPSC Code: 41106300

Propriétés

• form: solution
• Quality Segment: 300
• usage: sufficient for ≥600 reactions
• feature: PCR Additives
• concentration: 100 ×
• technique(s): PCR: suitable, whole genome amplification: suitable
• color: red
• input: crude DNA
• solubility: water: soluble
• storage temp.: 2-8°C

Description

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:

• in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
• as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
• for analyzing the degree of cellular DNA contamination by qPCR

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

Informations sur la sécurité

• Pictogrammes: GHS07,GHS09
• Mot-clé: Warning
• Codes de danger: H315,H317,H319,H410
• Mentions de précaution: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Classe de stockage: 12 - Non Combustible Liquids
• Que se passe-t-il ?: WGK 3
• EPI (équipement de protection individuelle): Eyeshields, Gloves