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Key Documents

PROTSIL2

Sigma-Aldrich

ProteoSilver Plus Silver Stain Kit

Synonyme(s) :

protein stain, silver stain

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About This Item

Code UNSPSC :
41105322
Nomenclature NACRES :
NA.32

Utilisation

 kit sufficient for 25 mini-gels (10 × 10 cm)

Technique(s)

protein staining: suitable

Température de stockage

20-25°C

Description générale

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.

Application

ProteoSilver Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.

Caractéristiques et avantages

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Informations légales

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3

Organes cibles

Eyes,Central nervous system, Respiratory system

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

149.0 °F - closed cup

Point d'éclair (°C)

65 °C - closed cup


Certificats d'analyse (COA)

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Articles

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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