The stability of this product at -80 °C has not been determined. This product is shipped on wet ice, and storage at -20 °C is recommended. The product is assigned a retest period of 2 years from the date of quality release. Refrigeration at 2-8 °C is acceptable for short term storage. Please see the link below to review additional information available in the product datasheet:https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/256/pic0005-pis-ms.pdf
PIC0005
Protease Inhibitor Cocktail
ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, For plant cell and tissue extracts, Non-freezing solution
Synonyme(s) :
Protease inhibitor cocktail
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A propos de cet article
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
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ReadyShield® Protease Inhibitor Cocktail, For plant cell and tissue extracts, Non-freezing solution
Quality Segment
description
Plant Cell and Tissue Extracts
form
ready-to-use solution
quality
200
packaging
pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
input
plant, fungi and insect sample(s)
solubility
water: soluble
storage temp.
−20°C
General description
This protease inhibitor cocktail has been optimized and tested for plant cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from various plant tissues. Whole extracts of plant seedlings from pea (Pisum sativum), bean (Phaseolus vulgaris), wheat (Triticum aestivum), tobacco (Nicotiana tobaccum), and arabidopsis (Arabidopsis thaliana) have been tested. Extracts of leaves or roots from pea, wheat, and tobacco have also been tested.This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0005 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P9599
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- Tailored for Plant cell and tissue extracts.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 30 g (wet weight) of various plant tissues.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
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Cet article | |||
|---|---|---|---|
| description For plant cell and tissue extracts, Non-freezing solution | description Safe for Proteomic Mass Spectrometry applications | description non-freezing solution | description for plant cell and tissue extracts, DMSO solution |
| form ready-to-use solution | form ready-to-use solution | form ready-to-use solution | form DMSO solution |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| packaging pkg of 5 mL (in amber polypropylene vial), pkg of 1 mL (in amber polypropylene vial) | packaging - | packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging - |
| input plant, fungi and insect sample(s) | input general use sample(s) | input mammalian sample(s) | input plant, fungi and insect sample(s) |
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Classe de stockage
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hello. Can I store it at -80C? thank you
1 answer-
Helpful?
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Do ReadyShield® inhibitor cocktails include EDTA?
1 answer-
No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.
Helpful?
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What is the recommended storage temperature for ReadyShield® products?
1 answer-
The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).
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