PHG0005

BIS-TRIS propane

• Synonyme(s) : 1,3-Bis[tris(hydroxyméthyl)méthylamino]propane
• Formule linéaire : CH₂[CH₂NHC(CH₂OH)₃]₂
• Numéro CAS: 64431-96-5
• Poids moléculaire : 282.33
• EC Number: 264-899-3
• UNSPSC Code: 12161705
• NACRES: NA.21
• Beilstein/REAXYS Number: 1786109
• MDL number: MFCD00004689

Propriétés

• biological source: synthetic
• Quality Segment: 400
• form: powder
• technique(s): cell culture | mammalian: suitable, pH adjustment: suitable
• impurities: Endotoxin, microbial, and trace metals; tested
• useful pH range: 6.3-9.5
• pKa (25 °C): (1) 6.8, (2) 9.0
• mp: 164-165 °C (lit.)
• suitability: suitable for manufacturing use
• application(s): downstream processes; pharma/biopharma processes
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• SMILES string: OCC(CO)(CO)NCCCNC(CO)(CO)CO
• InChI: 1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
• InChI key: HHKZCCWKTZRCCL-UHFFFAOYSA-N

Description

General description

Our SAFC^® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.

A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable