P9120

PNGase F from Elizabethkingia meningoseptica

recombinant, expressed in E. coli, set of 100 units nanomolar unit

• Synonyme(s) : N-Glycosidase F, PNGase F, Peptide N-glycosidase
• Numéro CAS: 83534-39-8
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numéro CE : 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD18839214
• Specific activity: ≥10 units/mg protein
• Recombinant: expressed in E. coli

Propriétés

• recombinant: expressed in E. coli
• Quality Segment: 200
• conjugate: (N-linked)
• specific activity: ≥10 units/mg protein
• mol wt: 36 kDa
• packaging: set of 100 units nanomolar unit
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation

• of recombinant soybean agglutinin (rSBA) in Nicotiana benthamiana (NbrSBA) and Solanum tuberosum (StrSBA)
• of frontal cortical lysate to verify the glycosylation profile of β-secretase (BACE proteins)
• of cell lysate for evaluating the siRNA silencing of cellular prion protein (PrP^c) post transfection

PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins.

Produit utilisé pour la déglycosylation des protéines.

Biochem/physiol Actions

PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-linked core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F.

Clive un glycane entier d′une glycoprotéine, à condition que le fragment d′asparagine glycosylé soit substitué sur ses extrémités amino et carboxyle avec une chaîne polypeptidique.

Packaging

Each set includes enzyme, two formulations of 5× reaction buffer (for routine and mass spectrometry downstream analysis), detergent and denaturation solutions

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Legal Information

N-Glycanase is a registered trademark of Agilent Technologies Inc

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08,GHS05,GHS09
• Mot-clé: Danger
• Mentions de précaution: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
• Classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Que se passe-t-il ?: WGK 3
• Codes de danger: H302,H311,H315,H317,H318,H334,H361f,H410