This supplement offers a total amine concentration, at 1000x, of 423 mg/liter. The components and concentration of each are as follows:
1,4-diamino butane dihydrochloride (known as putrescine dihydrochloride) - 0.005 % or 50 mg/L
Spermine free base - 0.0048 % or 48 mg/L
Spermidine free base - 0.0025 % or 25 mg/L
Ethanolamine - 0.03 % or 300 mg/L
P8483
Polyamine Supplement (1000×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonyme(s) :
Polyamine cell culture tested
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A propos de cet article
NACRES:
NA.75
UNSPSC Code:
12352205
Le tarif et la disponibilité ne sont pas disponibles actuellement.
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Laissez-nous vous aiderbiological source
non-animal source
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
General description
Polyamine Supplement (1000×) is a general medium supplement specially formulated to boost cell growth for enhanced viral and protein expression. One mL supplement is recommended for 1 liter of medium.
Application
Polyamine Supplement (1000×) has been used with serum-free medium (SFM) to culture human lymphocytes from peripheral blood mononuclear cells (PBMCs).[1] It has also been used in combination with antioxidant supplement (A 1345) to culture zygotes.
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Cet article | |||
|---|---|---|---|
| form liquid | form liquid | form solution | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source non-animal source | biological source - | biological source - | biological source - |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| shipped in ambient | shipped in ambient | shipped in - | shipped in dry ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Min Kyoung Jeon et al.
BMC biotechnology, 10, 70-70 (2010-09-22)
Serum-containing medium (SCM), which has a number of poorly defined components with varying concentrations, hampers standardization of lymphocyte cultures. In order to develop a serum-free medium (SFM) for the expansion of human lymphocytes from peripheral blood mononuclear cells (PBMCs), a
Effect of three oxygen levels in culture atmosphere and addition of commercial antioxidant in the development of bovine embryos produced in vitro
Delgado TGA
Acta Universitaria, 28, 53-57 (2018)
Yu Chen et al.
Nature methods, 18(5), 528-541 (2021-05-05)
Human pluripotent stem cells (hPSCs) are capable of extensive self-renewal yet remain highly sensitive to environmental perturbations in vitro, posing challenges to their therapeutic use. There is an urgent need to advance strategies that ensure safe and robust long-term growth
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| P8483-5ML | 04061834401483 |
| P8483-100ML | 04061837036316 |
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What polyamines are present in the supplement and at what concentration?
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