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P8045

Protein G-Biotin from Streptococcus sp.

Name: Protein G-Biotin from <I>Streptococcus</I> sp.
Brand: SIGMA

recombinant, expressed in unspecified host, lyophilized powder

Synonyme(s) :

Biotinylated Protein G, Protein G Biotin, Protein G-Biotin

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A propos de cet article

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00132111

Form:

lyophilized powder

Recombinant:

expressed in unspecified host

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Propriétés

recombinant

expressed in unspecified host

Quality Segment

200

conjugate

biotin conjugate

form

lyophilized powder

composition

Protein, ~90% biuret

extent of labeling

2-4 mol d-biotin per mol protein

technique(s)

direct ELISA: 1:70,000 using human IgG

storage temp.

2-8°C

Description

General description

Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.

Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

Application

Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.

Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.

Biochem/physiol Actions

Binds IgG only from most mammalian species except cat; binds chicken IgG.

Packaging

Package size based on protein content

Preparation Note

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

Other Notes

Truncated - lacks albumin, Fab and membrane binding sites.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Cet article	P8170	P3101	P8651
Sigma-Aldrich P8045 Protein G-Biotin from Streptococcus sp.	Sigma-Aldrich P8170 Protein G−Peroxidase from Streptococcus sp.	Sigma-Aldrich P3101 Protein L from Peptostreptococcus magnus	Sigma-Aldrich P8651 Protein A–Peroxidase from Staphylococcus aureus/horseradish
description recombinant, expressed in unspecified host, lyophilized powder	description recombinant, expressed in unspecified host, lyophilized powder	description >95% (SDS-PAGE), recombinant, expressed in E. coli, essentially salt-free, lyophilized powder	description lyophilized powder
recombinant expressed in unspecified host	recombinant expressed in unspecified host	recombinant expressed in E. coli	recombinant -
technique(s) direct ELISA: 1:70,000 using human IgG	technique(s) direct ELISA: 1:100,000 using human IgG	technique(s) indirect ELISA: 1:100,000	technique(s) direct ELISA: 1:80,000 using human IgG
form lyophilized powder	form lyophilized powder	form essentially salt-free, lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200, 100
conjugate biotin conjugate	conjugate peroxidase conjugate	conjugate unconjugated	conjugate peroxidase conjugate

Classe de stockage

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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