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P7936

Phosphocreatine disodium salt hydrate

≥97%

Synonyme(s) :

Creatine phosphate disodium salt, N-(Imino[phosphonoamino]methyl)-N-methylglycine

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A propos de cet article

Formule linéaire :
C4H8N3O5PNa2 · x H2O
Numéro CAS:
Poids moléculaire :
255.08 (anhydrous basis)
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
213-074-6
MDL number:
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Nom du produit

Phosphocreatine disodium salt hydrate, ≥97%

Quality Segment

assay

≥97%

form

powder

impurities

≤0.02% free creatine

color

white

solubility

water: 50 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].CN(CC(O)=O)C(=N)NP([O-])([O-])=O

InChI

1S/C4H10N3O5P.2Na.H2O/c1-7(2-3(8)9)4(5)6-13(10,11)12;;;/h2H2,1H3,(H,8,9)(H4,5,6,10,11,12);;;1H2/q;2*+1;/p-2

InChI key

KZVYPOKBYRXZKS-UHFFFAOYSA-L

Application

Phosphocreatine disodium salt hydrate has been used:
  • as a component: of Xenopus buffer (XB) buffer to remove the membrane-bound proteins[1]
  • of M5+ buffer to perform polarized total internal reflection fluorescence (polTIRF) experiments [2]
  • of ATP regenerating system for 7-nitro-2-1,3-benzoxadiazol-4-yl phospholipid(NBD-PL) translocation assay[3]

Biochem/physiol Actions

Phosphocreatine disodium salt hydrate is useful to study nasopharyngeal carcinoma cells and induced temporary cardiac arrest. It also evaluate the effects of adenosine A(2A) receptor modulation in the nucleus accumbens on cocaine seeking in rats.
Phosphocreatine is widely known for its quickest form of regeneration using enzyme creatine kinase. It functions as a temporal energy buffer. Phosphocreatine is present in muscle tissues and plays a major role in maintaining energy homeostasis in muscle cells.[4]
Phosphocreatine, a high-energy phosphate reservoir in vertebrate and some invertebrate muscles, provides phosphate for ADP-ATP conversion.

Preparation Note

Prepared enzymatically

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Cet article
P1937279202380
form

powder

form

powder

form

powder

form

crystalline

assay

≥97%

assay

≥95% (enzymatic)

assay

≥98.0% (NT)

assay

≥97% (TLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

water: 50 mg/mL, clear, colorless

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless

solubility

H2O: 0.1 g/mL, clear, colorless

solubility

water: 150 mg/mL

color

white

color

white

color

-

color

white

impurities

≤0.02% free creatine

impurities

-

impurities

≤0.6% free creatine

impurities

-


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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Certificats d'analyse (COA)

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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How to calulate the concentration(M) of this product? Because the Linear Formula is 4H8N3O5PNa2 · x H2O, it could not be calculted real molecular weight.

    1 answer
    1. The exact molar mass of this product is lot-specific but can be estimated using the reported water content determined by Karl Fischer analysis. The specification for water content is set between 22% and 28% as a weight percentage. For detailed assistance with determining degrees of hydration from reported water content, local Technical Service should be contacted directly.

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  4. Va bene come Fosfocreatina?

    1 answer
    1. The sodium form of phosphocreatine, in equimolar quantities, is comparable to the free form in biological activity. The sodium form demonstrates a higher solubility rate in water. Certain applications may require a specific form, which would have to be determined by the end user.

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