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P6407

Poly-ᴅ-Lysine Hydrobromide

Name: Poly-ᴅ-Lysine Hydrobromide
Brand: SIGMA

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Synonyme(s) :

PDL

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A propos de cet article

Formule linéaire :

D-Lys-(D-Lys)_n-D-Lys · xHBr

Numéro CAS:

27964-99-4

UNSPSC Code:

12352202

PubChem Substance ID:

24898764

NACRES:

NA.75

MDL number:

MFCD00131934

Biological source:

synthetic (organic)

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Concentration:

0.016—0.032 mmol lysine

Mol wt:

70,000-150,000

Le tarif et la disponibilité ne sont pas disponibles actuellement.

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Propriétés

Nom du produit

Poly-D-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

biological source

synthetic (organic)

Quality Segment

200

sterility

γ-irradiated

product line

BioReagent, BioXtra

form

lyophilized powder

mol wt

70,000-150,000

packaging

pkg of 10 X 5 mg, pkg of 5 mg

concentration

0.016—0.032 mmol lysine

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm²

impurities

<10% water (by Karl Fischer)

solubility

H₂O: soluble 50 mg/mL, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

Description

Application

Le bromhydrate de poly-D-lysine a été utilisé :

pour enduire des plaques destinées à la culture de cellules précurseurs gliales de rat (RGPCS).
pour enduire des fioles et des boîtes pour la culture de tanycytes.
pour préparer la surface de lamelles couvre-objet et faciliter l'adhésion cellulaire.

Les polymères de poly-D-lysine peuvent servir à préparer des surfaces favorisant l'adhérence cellulaire. Les polymères de D-lysine peuvent également être utilisés avec des cellules qui digèrent les polymères de poly-L-lysine et induisent une absorption excessive de L-lysine.

Il est recommandé d'utiliser ce produit comme substrat de culture cellulaire, à raison de 0,5 à 1,0 ml de solution à 0,1 mg/ml pour 25 cm². Les versions de faible poids moléculaire sont moins visqueuses, mais les versions de poids moléculaire plus élevé offrent davantage de points d'attache par molécule.

Biochem/physiol Actions

Le bromhydrate de poly-D-lysine (PDL) est un facteur d'attachement cellulaire non spécifique qui favorise l'adhésion des cellules aux substrats solides en augmentant les interactions électrostatiques entre les ions chargés négativement de la membrane cellulaire et la surface de culture. Une fois adsorbée sur la surface de culture, la poly-D-lysine augmente le nombre de sites de fixation cellulaire de charge positive.

Features and Benefits

Produit stérilisé par rayonnement gamma (γ).
Les solutions stériles de poly-D-lysine peuvent être conservées pendant trois ans entre 2 et 8 ˚C.

Preparation Note

Ce produit a un poids moléculaire de 70 000-150 000, est testé sur des cultures cellulaires et est stérilisé par rayonnement gamma. Pour éliminer le HBr, dissoudre le produit dans un tampon neutre et réaliser une dialyse pour retirer les sels. D′une manière générale, pour utiliser ce produit comme facteur de fixation, ajouter 50 ml d′eau stérile de qualité « culture tissulaire » à 5 mg de poly-lysine, et recouvrir en conditions aseptiques la surface avec 1 ml de solution pour 25 cm². Après 5 minutes, retirer la solution par aspiration et rincer soigneusement la surface. Laisser sécher deux heures avant introduction des cellules et du milieu.

Other Notes

La poly-D-lysine est un polymère d'acide aminé à charge positive comportant environ un HBr par résidu lysine. Le bromhydrate permet de former une poudre cristalline de poly-D-lysine soluble dans l'eau. Une petite quantité de produit peut être repliée en structure bêta, car HBr perturbe la formation de liaisons hydrogène entre les groupements amine et soit les groupements carboxyle soit les fractions contenant des atomes d'azote ou d'oxygène.

Disclaimer

Les solutions stériles sont stables pendant 2 ans maximum lorsqu'elles sont conservées entre 2 °C et 8 °C. À conserver déshydraté à -20 °C.

Comparer avec des articles similaires

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1 of 1

Cet article	P7886	P6403	P0899
Sigma-Aldrich P6407 Poly-D-lysine hydrobromide	Sigma-Aldrich P7886 Poly-D-lysine hydrobromide	Sigma-Aldrich P6403 Poly-D-lysine hydrobromide	Sigma-Aldrich P0899 Poly-D-lysine hydrobromide
description mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture	description mol wt 30,000-70,000	description mol wt 4,000-15,000	description mol wt 70,000-150,000
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility γ-irradiated	sterility -	sterility -	sterility -
biological source synthetic (organic)	biological source -	biological source -	biological source -
concentration 0.016—0.032 mmol lysine	concentration -	concentration -	concentration -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
mol wt 70,000-150,000	mol wt 30,000-70,000	mol wt 4,000-15,000	mol wt 70,000-150,000

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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

1–10 of 11 Questions

2 years ago

이 가루는 3차 증류수에 녹이면 될까요? 녹인 PDL은 -20에 보관하면 되나요?

1 answer
1. Technical Support
  
  2 years ago
  
  This product is tested for solubility in water at 50 mg/mL. The product, in solution, is quite stable when stored at 2-8°C. The freeze/thaw stability of this material has not been investigated. However, the thawing process may cause degradation. The product P4832 is a prepared solution that is stored refrigerated and assigned a 2 year shelf life.
  Please see the link below to review the product datasheet for additional information:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/784/704/p6407pis.pdf
  
  Helpful?
Anonymous

2 years ago

Comparitively which is better to use with Human embryonic kidney cells, P6407 or P2636?

1 answer
1. Technical Support
  
  2 years ago
  
  Comparison testing of these products on Human embryonic kidney cells has not been conducted. In general, P6407 is preferred due to the fact that P2636, provided in L form, is more susceptible to degradation by proteases secreted by cell lines compared to the d-form polymer provided in P6407.
  
  Helpful?
4 years ago

Is there a standard method of determining the quality of the coating? (Particular focus should be placed on ensuring that the cells adhere as evenly as possible)

1 answer
1. Technical Support
  
  4 years ago
  
  As mentioned on the product detail page, this product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high molecular weight versions provide more attachment sites per molecule.
  For more information on using this product for quality coating, please read through the product information sheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/784/704/p6407pis.pdf
  
  Helpful?
Anonymous

4 years ago

Does Product P6407, Poly-D-lysine hydrobromide, need to be dialyzed to remove the hydrogen bromide?

1 answer
1. Technical Support
  
  4 years ago
  
  The hydrogen bromide will not interfere with the cell growth. The hydrogen bromide salts are used to make our poly-L-lysine solutions that are cell culture tested. For these products, there is no interference in cell growth from the bromide.
  
  Helpful?
Anonymous

4 years ago

How do I determine which molecular weight range of poly-lysine to use?

1 answer
1. Technical Support
  
  4 years ago
  
  Poly-lysine is used to coat surfaces to increase the charge present. The lower molecular weight poly-L-lysine (30-70 kD) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kD) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kD. Please check the literature for information on the molecular weight range used for your cell type.
  
  Helpful?
Anonymous

4 years ago

Do you have a protocol for coating cell cultureware or glass coverslips/slides with Product P6407, Poly-D-Lysine?

1 answer
1. Technical Support
  
  4 years ago
  
  The protocol for coating surfaces with poly-D-lysine is the same as for poly-L-lysine. The optimal conditions must be determined for each cell line and application. In general, the following steps can be used.1. Add 50 ml of sterile tissue culture grade water to 5 mg of poly-D-lysine.2. Aseptically coat culture surface with 0.5-1.0 ml of solution per 25 cm2. Rock gently to ensure even coating of the culture surface.3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.4. Allow to dry at least two hours before introducing cells and medium.
  
  Helpful?
Anonymous

4 years ago

What protocol should I follow for coating Transwells with Product P6407, Poly-D-lysine dihydrochloride?

1 answer
1. Technical Support
  
  4 years ago
  
  The same protocol can be followed for coating plates with poly-lysine. The transwell membrane is immersed in the poly-lysine solution.
  
  Helpful?
Anonymous

4 years ago

What is the Department of Transportation shipping information for this product?

1 answer
1. Technical Support
  
  4 years ago
  
  Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
  
  Helpful?
Anonymous

4 years ago

Why would I use Product P6407, Poly-D-lysine hydrobromide, instead of poly-L-lysine?

1 answer
1. Technical Support
  
  4 years ago
  
  Certain cells can digest poly-L-lysine, in which case the poly-D-lysine should be used.
  
  Helpful?
Anonymous

4 years ago

How long can I store poly-lysine coated slides or plates?

1 answer
1. Technical Support
  
  4 years ago
  
  Plates and/or slides can be stored after drying, protected from dust for up to 1 year. Inspect the plates/slides to make sure there are no cracks in the coating prior to use. Each lab should determine the maximum length of time for storage based on the application.
  
  Helpful?