P4932

Polymyxine B sulfate salt

powder, non-animal origin, suitable for cell culture, BioReagent

• Synonyme(s) : Polymixin, Polymixin B, Polymixin B sulfate
• Formule empirique (notation de Hill): C₅₅H₉₆N₁₆O₁₃ · 2H₂SO₄
• Numéro CAS: 1405-20-5
• Poids moléculaire : 1385.61
• Numéro CE : 215-774-7
• Numéro MDL: MFCD00131991
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.76

Propriétés

• Nom du produit: Polymyxine B sulfate salt, powder, BioReagent, suitable for cell culture
• Gamme de produits: BioReagent
• Niveau de qualité: 200
• Forme: powder
• Puissance: ≥6,000 USP units per mg
• Technique(s): cell culture | mammalian: suitable
• Spectre d'activité de l'antibiotique: Gram-negative bacteria
• Mode d’action: cell membrane | interferes
• Température de stockage: 2-8°C
• Chaîne SMILES: O=C(C(NC(C(CCN)NC(CCCCC(C)CC)=O)=O)C(C)O)NC(CCN)C(NC(CCNC(C(NC(C(NC(C(CCN)N1)=O)CCN)=O)C(O)C)=O)C(NC(CCN)C(NC(CC2=CC=CC=C2)C(NC(CC(C)C)C1=O)=O)=O)=O)=O.O=S(O)(O)=O
• InChI: 1S/C48H82N16O13.H2O4S/c1-27(2)24-37-47(76)59-32(11-19-52)41(70)56-31(10-18-51)43(72)61-35(14-22-65)39(68)54-21-13-34(45(74)57-33(12-20-53)44(73)64-38(48(77)63-37)25-28-6-4-3-5-7-28)60-42(71)30(9-17-50)58-46(75)36(15-23-66)62-40(69)29(8-16-49)55-26-67;1-5(2,3)4/h3-7,26-27,29-38,65-66H,8-25,49-53H2,1-2H3,(H,54,68)(H,55,67)(H,56,70)(H,57,74)(H,58,75)(H,59,76)(H,60,71)(H,61,72)(H,62,69)(H,63,77)(H,64,73);(H2,1,2,3,4)
• Clé InChI: HNDFYNOVSOOGDU-UHFFFAOYSA-N

Description

Description générale

Polymyxin B is a member of the polymyxin group of antibiotics, obtained from different strains of Bacillus polymyxa and related species. It contains a cyclic heptapeptide unit and a tripeptide side chain, which is N-acylated by a fatty acyl residue. Polymyxin B sulfate salt is generally used for the treatment of Gram-negative bacterial infections.[1]
Chemical structure: peptide

Application

Polymyxin B sulfate salt has been used:

• for the treatment of BAC-1.2F5 murine macrophages as an LPS (lipopolysaccharide) inhibitor[2]
• as positive and negative control during microbicidal concentration (MCC) assay[3][4]

Actions biochimiques/physiologiques

Mode d'action : Le sulfate de polymyxine B se lie à la partie lipide A des lipopolysaccharides bactériens†, ce qui altère la membrane cytoplasmique en induisant la formation de pores suffisamment grands pour provoquer une fuite de nucléotides dans les parois bactériennes. Ceci modifie la perméabilité de la membrane cytoplasmique.

Spectre antimicrobien : Présente une action bactéricide vis-à-vis de la plupart des bacilles à Gram négatif††, notamment E. Coli et la plupart des champignons et des bactéries à Gram positif.

Polymyxin B sulfate has been used clinically to treat infections of the urinary tract, meninges and blood stream caused by susceptible strains of Pseudomonas aeruginosa. It also has been used in studying multidrug-resistant pathogens, as an immobilized agent for removal of endotoxins, and to induce pore formation in the membranes of cortex cells from excised sorghum roots.

Attention

As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.

Notes préparatoires

Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

Autres remarques

1mu,5mu

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302
• Conseils de prudence: P264 - P270 - P301 + P312 - P501
• Classification des risques: Acute Tox. 4 Oral
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves