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P1473

Sigma-Aldrich

PCR 100 bp Low Ladder

for electrophoresis of PCR fragments

Synonyme(s) :

100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

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About This Item

Code UNSPSC :
41141827
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

200

Forme

liquid

Utilisation

75 uses

Adéquation

suitable for electrophoresis (DNA)

Température de stockage

−20°C

Description générale

Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

Application

PCR 100 bp Low Ladder has been used:
  • in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis
  • to visualize DNA from enzyme digested PCR mixture
  • to estimate the molecular weight of the PCR product fragments

Composants

Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Autres remarques

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.
For optimal resolution, the recommended agarose gel concentration is 2.0%.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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The relationships between performance traits and the bGH/Alu I and Pit-1/Hinf Ipolymorphisms in Holstein cows
Ozdemir M, et al.
Indian Journal of Animal Sciences (India) , 52, 186-191 null
Patricia Rivera et al.
Scientific reports, 10(1), 21154-21154 (2020-12-05)
Obesity has been firmly established as a major risk factor for common disease states including hypertension, type 2 diabetes mellitus, and chronic kidney disease. Increased body mass index (BMI) contributes to the activation of both the systemic and intra-tubular renin
Application of zinc oxide nanoparticles and plant growth promotingbacteria reduces genetic impairment under salt stress in tomato (Solanum lycopersicum L.`Linda?)
Hosseinpour A, et al.
A?r? : A?r? (Algoloji) Derne?i'nin Yay?n organ?d?r = The Journal of the Turkish Society of Algology, 10, 521-521 null
Gbenga A Adewumi et al.
Frontiers in microbiology, 3, 436-436 (2013-01-15)
In this study, bacterial composition of iru produced by natural, uncontrolled fermentation of Parkia biglobosa seeds was assessed using culture-independent method in combination with culture-based genotypic typing techniques. PCR-denaturing gradient gel electrophoresis (DGGE) revealed similarity in DNA fragments with the
Susan Walker et al.
PloS one, 9(9), e106262-e106262 (2014-09-04)
In human adults, and animals, the Apelin-APJ ligand-receptor system is emerging as having a role in the pathogenesis of cardiovascular function and heart failure. The aim was to investigate expression, and regulation by oxygen, of the Apelin APJ receptor (APJ)

Articles

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

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