P0114

Phosphatase, Alkaline from bovine intestinal mucosa

BioUltra, ≥5,700 DEA units/mg protein

• Synonyme(s) : Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Numéro CAS: 9001-78-9
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• NACRES: NA.54
• Numéro CE : 3.1.3.1 (BRENDA, IUBMB)
• MDL number: MFCD00131849
• Specific activity: ≥5,700 DEA units/mg protein
• Assay: ≥10 mg/mL (biuret)
• Concentration: ≥10 mg/mL

Propriétés

• product line: BioUltra
• Quality Segment: 200
• assay: ≥10 mg/mL (biuret)
• form: (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
• specific activity: ≥5,700 DEA units/mg protein
• mol wt: dimer ~160 kDa
• concentration: ≥10 mg/mL
• technique(s): protein detection: suitable
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation.

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A.

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl₂ and 0.12 mM ZnCl₂, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Informations sur la sécurité

• Pictogrammes: GHS08
• Mot-clé: Danger
• Codes de danger: H334
• Mentions de précaution: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)