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Key Documents

O5003

Sigma-Aldrich

OPI Media Supplement

Hybri-Max™, γ-irradiated, powder, suitable for hybridoma

Synonyme(s) :

OPI Media for cell culture

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Qualité

Hybri-Max™

Niveau de qualité

200

Stérilité

γ-irradiated

Forme

powder

Concentration

18-40 mg/vial

Technique(s)

cell culture | hybridoma: suitable

Impuretés

endotoxin, tested

Température de stockage

−20°C

Application

OPI Media Supplement has been used to grow hybridoma cell lines from XenoMouse strains and BALB/c female mice.

Autres remarques

Formulated with: 0.15 g oxaloacetate, 0.05 g pyruvate, and 0.0082 g insulin.

Reconstitution

Reconstitute contents of vial with 10 mL sterile water. Do not use cell culture medium for initial reconstitution. Each vial sufficient to prepare 1 liter of medium. Final working concentration: 1 mM oxaloacetate, 0.45 mM pyruvate, 0.2 U/ml insulin.

Informations légales

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

CorrosionExclamation mark
GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H314,H317

Classification des risques

Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Jun Yang et al.
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Neurogranin (NRGN) is a small neuronal protein that plays an important role in synaptic signaling by regulating calmodulin (CaM) availability. In this study, we developed an ELISA to measure NRGN quantitatively in serum samples from a cohort of acute traumatic
Gabriela A Canziani et al.
Analytical biochemistry, 325(2), 301-307 (2004-01-31)
Experimental and data analysis protocols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface plasmon resonance biosensor platforms. The screening methods involved capturing antibodies from crude supernatants using Fc-specific antibody surfaces and monitoring antigen binding at a
Tzu-Ling Sung et al.
PLoS pathogens, 5(1), e1000263-e1000263 (2009-01-17)
Cyclin T1 is a regulatory subunit of a general RNA polymerase II elongation factor known as P-TEFb. Cyclin T1 is also required for Tat transactivation of HIV-1 LTR-directed gene expression. Translation of Cyclin T1 mRNA has been shown to be
Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
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