NA0600

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit

1 kit sufficient for 5 preparations

• Synonyme(s) : Gen Elute, GenElute Endotoxin-Free Plasmid Kit
• Code UNSPSC : 41105501
• Nomenclature NACRES : NA.52

Propriétés

• Utilisation: kit sufficient for 5 preparations
• Caractéristiques du produit alternatif plus écologique: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Autre catégorie plus écologique: , Aligned
• Température de stockage: 15-25°C

Description

Description générale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Application

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Caractéristiques et avantages

• Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation required

Autres remarques

For additional information, please see www.sigma-aldrich.com/genelutehp.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Pictogrammes: GHS02,GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H226,H290,H302 + H332,H315,H319,H334,H336,H372
• Conseils de prudence: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• Organes cibles: Central nervous system, Lungs
• Code de la classe de stockage: 3 - Flammable liquids
• Point d'éclair (°F): 77.0 °F - closed cup
• Point d'éclair (°C): 25 °C - closed cup