N6658

Nutrient Mixture F-12 Ham

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonyme(s) : Ham’s F-12
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217417

Propriétés

• Nom du produit: Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: yes; HEPES: no; phenol red: yes; sodium pyruvate: 0.11 g/L; NaHCO₃: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Listen to an Audio Overview
Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.

Application

Nutrient Mixture F-12 Ham has been used:

• to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
• to culture LAN-1 cells
• to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
• with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• Que se passe-t-il ?: WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable