N2770

p-Nitrophenyl phosphate

SIGMAFAST^™, chromogenic, tablet

• Synonyme(s) : pNPP tablets
• UNSPSC Code: 12352204
• NACRES: NA.83
• EC Number: 224-246-5
• MDL number: MFCD00284586

Propriétés

• Nom du produit: SIGMAFAST^™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL
• Quality Segment: 200
• form: tablet
• solubility: water: 0.25 tablet/mL, clear, colorless
• storage temp.: −20°C

Description

Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST^™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Preparation Note

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable