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MX555S100

Sigma-Aldrich

Mix-n-Stain CF 555 Antibody Labeling Kit (50-100μg)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Forme

liquid

Niveau de qualité

Conditions de stockage

protect from light

Fluorescence

λex 555 nm; λem 565 nm

Température de stockage

−20°C

Description générale

This kit contains everything you need to rapidly label an antibody. The labeling procedure comprises simple mixing of your antibody and the optimally formulated dye in the reaction buffer provided, followed by a brief incubation. The resulting solution is ready for staining without further purification. The kit is suitable for labeling commercially available primary antibodies, either directly or after a simple antibody clean-up step. After labeling, the dye is covalently linked to the antibody.
This labeling kit is for labeling 50-100 μg antibody with the red fluorescent dye CF555. Mix-n-Stain CF dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF555 is an orange-red fluorescent dye and one of an outstanding series of CF dyes developed by Biotium. CF555 is spectrally similar to Alexa Fluor 555 and Cy®3 dyes. CFdyes are superior to Alexa Fluor dyes and Cy® dyes for antibody labeling by having combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging.

Application

The Mix-n-Stain CF dye antibody labeling kit can be used to label primary antibodies targeted towards specific peptides, for ELISA, western blot, immunohistochemistry, flow cytometry, immunofluorescence or immunocytochemistry applications.

Caractéristiques et avantages

A major advantage of the labeling kits is the superior CF dyes, many of which are brighter and more photostable than Alexa Fluor dyes and other similar commercial dyes. With a large list of CF dyes of wavelengths covering the entire visible and near-infrared spectra, you have unprecedented freedom to label any commercial primary antibody with a color of your choice, making multi-color detection simple and affordable.
Mix-n-Stain CF dye antibody labeling kits dramatically simplify the process of preparing fluorescently labeled antibodies, particularly primary antibodies. Simply mix your antibody with a CF dye of your choice in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a fluorescent antibody conjugate that is as good as the best pre-labeled fluorescent antibody you can buy from some of the leading suppliers. There is no need to calculate how much dye you should use; just mix your antibody with the entire amount of dye provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA.

Composants

The kit consists of 1 vial each of Mix-n-Stain Dye, Mix-n-Stain Reaction Buffer (10x), Mix-n-Stain Storage Buffer, and an ultrafiltration vial.

Remarque sur l'analyse

Direct replacement for: Alexa Fluor 555, ATTO 550, Cy®3, DY-547, DyLight®549, TRITC

Informations légales

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
CF is a trademark of Biotium, Inc.
Cy is a registered trademark of Cytiva
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Mix-n-Stain is a trademark of Biotium, Inc.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Chronic 3 - Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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He W, et al.
Journal of Virology, 8866-8874 (2016)
PyMINEr Finds Gene and Autocrine-Paracrine Networks from Human Islet scRNA-Seq
Tyler SR, et al.
Testing, 26(7), 1951-1964 (2019)
Scott R Tyler et al.
Cell reports, 26(7), 1951-1964 (2019-02-14)
Toolsets available for in-depth analysis of scRNA-seq datasets by biologists with little informatics experience is limited. Here, we describe an informatics tool (PyMINEr) that fully automates cell type identification, cell type-specific pathway analyses, graph theory-based analysis of gene regulation, and
Paraoxonase 2 decreases renal reactive oxygen species production, lowers blood pressure, and mediates dopamine D(2) receptor-induced inhibition of NADPH oxidase.
Yang, Y., et al.
Free Radical Biology & Medicine, doi: 10-doi: 10 (2012)
Jun Xu et al.
eLife, 11 (2022-01-26)
Expansion of the available repertoire of reagents for visualization and manipulation of proteins will help understand their function. Short epitope tags linked to proteins of interest and recognized by existing binders such as nanobodies facilitate protein studies by obviating the

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