MAK452

Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

Sufficient for 100 colorimetric tests

• Synonyme(s) : G6PDH Inhibitor Screening Kit
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 100
• Méthode de détection: colorimetric
• Maladie(s) pertinente(s): hematological disorder, anemia; cancer
• Température de stockage: −20°C

Description

Description générale

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.

Application

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit may be used for:

• Cancer Research
• Drug Discovery

Caractéristiques et avantages

• Efficient Inhibitor Screening: Our kit allows for efficient screening of G6PDH inhibitors using just 5 μL of test compound, minimizing effort while ensuring accurate results.
• Compatibility with High-Throughput Systems: Seamlessly integrating into high-throughput handling systems, our kit guarantees efficiency and accuracy, streamlining your screening processes.

Adéquation

The kit is suitable for high throughput inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

Principe

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅ nm. The increase in absorbance at A₅₆₅ nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

Autres remarques

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Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 12 - Non Combustible Liquids