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MAK320

Sigma-Aldrich

D-2-Hydroxyglutarate (D2HG) Assay Kit

sufficient for 200 fluorometric reactions

Synonyme(s) :

D2HG Test Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Utilisation

sufficient for 200 reactions (Fluorometric)

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cancer; neurological disorders

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

The level of D-2-Hydroxyglutarate (D2HG) is low in normal cells and tissues, but is significantly elevated in metabolic diseases, such as the rare autosomal disorder D2HG aciduria. D2HG is mildly elevated in other metabolic disorders including multiple acyl-CoA dehydrogenase deficiency, dihydrolipoyl dehydrogenase deficiency, pyruvate decarboxylase deficiency and pyruvate carboxylase deficiency, various cancers, and in neoplasms with mutations in the isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) genes. Detection of elevated D2HG is an important biomarker for early diagnosis, prognosis, monitoring and the development of therapeutic strategies against these diseases.

Caractéristiques et avantages

  • Highly sensitive and rapid enzymatic assay for the detection of D2HG levels in various biological fluids
  • Supportive calculator (Click here to download a calculator excel file) Analyze results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 200 tests
  • Detection time: only 30-60 minutes

Adéquation

Suitable for the detection of D2HG levels in cells and tissues lysates, serum, urine, cultured cells and culture supernatants.

Principe

Developed in partnership with the German Cancer Research Center (DKFZ), the D2HG Assay Kit is a rapid and sensitive enzymatic assay for the detection of D2HG levels in various biological fluids: serum, urine, cell culture supernatants, and cell or tissue lysates. The assay, originally developed by Balss et al., 8 is based on the oxidation of D2HG to α-ketoglutarate (αKG) by the enzyme (D)-2-hydroxyglutarate dehydrogenase (HGDH) coupled to the reduction of NAD+ to NADH (see Figure 1). The amount of NADH formed is then quantitated by the diaphorase mediated reduction of resazurin to the fluorescent dye resorufin (λex = 540 nm/λem = 590 nm).

Informations légales

This product is sold under license from the German Cancer Research Center (DKFZ) and University Clinic of Heidelberg. Use of this product is covered by certain US and foreign patents, including US Pat. No. 9,487,815, EP Pat. No. 2 820 145 and foreign equivalents. Use of this product is for research purposes only.

Code de la classe de stockage

11 - Combustible Solids

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Certificats d'analyse (COA)

Lot/Batch Number
0000400892
0000400892
0000251415
0000202421

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Roberta Leonardi et al.
The Journal of biological chemistry, 287(18), 14615-14620 (2012-03-24)
Isocitrate dehydrogenase (IDH) is a reversible enzyme that catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (ICT) to α-ketoglutarate (αKG) and the NADPH/CO(2)-dependent reductive carboxylation of αKG to ICT. Reductive carboxylation by IDH1 was potently inhibited by NADP(+) and, to a
R A Chalmers et al.
Clinica chimica acta; international journal of clinical chemistry, 77(2), 117-124 (1977-06-01)
Detailed studies, using gas chromatography and mass spectrometric methods, of the urinary organic acids excreted by a patient with proven pyruvate decarboxylase deficiency are reported. In addition to the greatly-increased levels of lactate and pyruvate, marked elevation in the levels
S I Goodman et al.
Pediatric research, 14(1), 12-17 (1980-01-01)
When amino acids were infused at a rate of 4 g/kg/day, an infant with hypoglycemia, metabolic acidemia and chronic regurgitation showed hypersarcosinemia and excreted abnormal amounts of sarcosine, isovalerylglycine, isobutyrylglycine, alpha-methylbutyrylglycine, and beta-hydroxyisovaleric, glutaric, alpha-hydroxyglutaric, methylsuccinic, and alpha-hydroxyisobutyric acids in
Mahmoud A Bassal et al.
Nature communications, 13(1), 2614-2614 (2022-05-14)
The interaction of germline variation and somatic cancer driver mutations is under-investigated. Here we describe the genomic mitochondrial landscape in adult acute myeloid leukaemia (AML) and show that rare variants affecting the nuclear- and mitochondrially-encoded complex I genes show near-mutual
R A Chalmers et al.
Journal of inherited metabolic disease, 3(1), 11-15 (1980-01-01)
A patient with protein-losing gastroenteropathy and egg allergy has been shown to have a previously unrecognized organic aciduria, D-2-hydroxyglutaric aciduria. The observations made are consistent with an inherited metabolic disorder in the catabolism of 5-aminolaevulinate possibly due to deficient activity
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Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What is the recommended approach for measuring 2-HG levels in cell culture samples using the MAK320 detection kit if the sample volume is very small (i.e. 100 ul) and there is no access to equipment for accurately measuring pH in small volumes? Additionally, is the pH range critical for the experiment to work? Would a higher or lower pH result in decreased enzyme activity or render the experiment ineffective?

    1 answer

    1. The recommended solution is to use litmus paper to measure the pH of the sample. Another option is to use a special electrode designed for very small volumes, although this can be complicated and expensive. While the pH of the sample is crucial for test performance, the pH range is wide, making pH paper suitable and requiring only a few microliters of the sample.

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