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MAK171

Sigma-Aldrich

Colorimetric Biotin Assay Kit

sufficient for 200 colorimetric assays

Synonyme(s) :

Biotin Quantitation Kit

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About This Item

Numéro CE :
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.84

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

dermatological diseases; neurological disorders

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Biotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.

Adéquation

Suitable for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution

Principe

This Colorimetric Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes HABA (4′-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm.

Notes préparatoires

Storage and Stability: The kit is shipped on dry ice and storage at –20 °C, protected from light, is recommended.

Precautions and Disclaimer
: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Code de la classe de stockage

10 - Combustible liquids


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Consulter la Bibliothèque de documents

High-throughput biotinylation of proteins.
High Throughput Protein Expression and Purification, 185-198 (2009)
Site-specific biotinylation of purified proteins using BirA.
Fairhead M and Mark H
Site-Specific Protein Labeling, 171-184 (2015)
Imre Gaspar et al.
Bio-protocol, 8(5), e2750-e2750 (2018-03-05)
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using basic laboratory equipment to convert arrays of

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