MAK168
Pyrophosphate Assay Kit
sufficient for 200 fluorometric tests (Blue fluorescence)
Synonyme(s) :
High-Sensitivity Pyrophosphate Assay Kit
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UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84
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sufficient for 200 fluorometric tests (Blue fluorescence)
application(s)
cosmetics
food and beverages
detection method
fluorometric
storage temp.
−20°C
General description
Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.
Application
The Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.
Biochem/physiol Actions
The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =316/λem = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
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Danger
hcodes
Hazard Classifications
Eye Dam. 1
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F
flash_point_c
87 °C
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