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MAK052

Sigma-Aldrich

Dosage de l'activité ALAT

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Kit de dosage de l'activité alanine aminotransférase, Kit de dosage de l'activité transaminase glutamique-pyruvique sérique, Kit de dosage de l'activité ALAT, Kit de dosage de l'activité ALT

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

gastrointestinal diseases

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... GPT(2875)
mouse ... GPT(76282)
rat ... GPT(81670)

Description générale

L'alanine aminotransférase (ALAT), également appelée transaminase glutamopyruvique (TGP), est une enzyme dépendante du phosphate de pyridoxal qui catalyse le transfert réversible d'un groupement aminé d'une alanine vers un α-cétoglutarate, formant du pyruvate et du glutamate. L'ALAT est surtout présente dans le foie et le sérum, mais on la retrouve également dans d'autres tissus. Les lésions hépatocellulaires entraînent souvent une augmentation du taux d'ALAT sérique, si bien que ce taux peut servir d'indicateur de lésions hépatiques.

Application

Le kit de dosage de l'activité ALAT permet de déterminer l'activité alanine aminotransférase (ALAT) dans des échantillons de sérum.[1][2][3]

Caractéristiques et avantages

Produit compatible avec un système de manipulation à haut débit.

Adéquation

Utilisable pour la mesure de l'activité alanine aminotransférase (ALT) dans des échantillons biologiques (tissus, cellules, sérum)

Principe

Le kit de dosage de l'activité ALAT offre un moyen simple et direct de mesurer l'activité ALAT dans différents échantillons biologiques.

L'activité ALAT est déterminée au moyen d'un dosage enzymatique couplé qui permet d'obtenir un produit mesurable par colorimétrie (570 nm)/fluorimétrie (λex = 535/λem = 587 nm) dont la quantité est proportionnelle à celle du pyruvate produit. Une unité d'ALAT équivaut à la quantité d'enzyme produisant 1,0 μmol de pyruvate par minute à 37 °C.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

92210

Timestrip Plus™ 0 °C

06693

Timestrip Plus™ -20 °C

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334,H412

Classification des risques

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8L23K07520
8L18K07520
8A24K07520
8K11K07520
8K03K07520

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Brett Palaschak et al.
Molecular therapy. Methods & clinical development, 5, 142-152 (2017-05-10)
Multiple independent adeno-associated virus (AAV) gene therapy clinical trials for hemophilia B, utilizing different AAV serotypes, have reported a vector dose-dependent loss of circulating factor IX (FIX) protein associated with capsid-specific CD8
Investigation of Therapeutic Effects of $\alpha$-Mangostin on Thioacetamide-Induced Cirrhosis in Rats
Nilbunga S.
Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 98(9), S91-S97 (2015)
Nan Wu et al.
Laboratory investigation; a journal of technical methods and pathology, 97(7), 843-853 (2017-06-06)
The Hippo signaling pathway and the Notch signaling pathway are evolutionary conserved signaling cascades that have important roles in embryonic development of many organs. In murine liver, disruption of either pathway impairs intrahepatic bile duct development. Recent studies suggested that
Melissa F Brereton et al.
Nature communications, 7, 13496-13496 (2016-11-25)
Insulin secretion from pancreatic β-cells is impaired in all forms of diabetes. The resultant hyperglycaemia has deleterious effects on many tissues, including β-cells. Here we show that chronic hyperglycaemia impairs glucose metabolism and alters expression of metabolic genes in pancreatic
Resolvin D1 and E1 alleviate the progress of hepatitis toward liver cancer in long-term concanavalin A-induced mice through inhibition of NF-KB activity.
Kuang H, et al.
Oncology Reports, 35(1), 307-317 (2016)
Afficher tous les articles scientifiques apparentés

Questions

1–8 of 8 Questions  

  1. Hi, Does this ALT kit depend on LDH for the ultimate readout? Our goal is to measure liver dysfunction in serum of mice. Would differences in serum LDH influence the results? Thanks Ricky

    1 answer

    1. This kit does not use lactate dehydrogenase (LDH) itself, but the measured results are proportional to the concentration of pyruvate generated. Because LDH (in the presence of NADH) can influence the concentration of pyruvate present, the presence of LDH in a sample may influence the assay results.

      LDH was not assessed in the development of this kit, but the kit has been used to test serum samples directly. It is recommended to perform several sample dilutions to determine the optimal sample concentration and ensure measured values fall within the linear range of the standard curve. Liver cell and liver-derived cell line lysates are more commonly measured, as serum levels of ALT are normally low. Regardless, considering the effect of LDH on pyruvate in the presence of NADH, a variable concentration of LDH and/or NADH may impact the ALT assay results.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. Can I know the specific activity of this ALT mix sample? In unit of U/mg

    1 answer

    1. The ALT Enzyme Mix included in this kit is a lyophilized mixture of enzymes. The components include Pyruvate Oxidase, Cocarboxylase, and Sucrose. The concentration and activity of each enzyme are considered proprietary. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/731/802/mak052bul.pdf

      Helpful?

  5. Is the ALT activity assay kit MAK052 compatible with EDTA plasma? Does EDTA interfere with the activity of the assay?

    1 answer

    1. EDTA has the potential to deactivate the enzyme. ​Therefore, it is recommended not to use samples containing EDTA for performing the AST and ALT assay kits, including the MAK055 kit.

      Helpful?

  6. How does the presence of pyruvate in the Krebs buffer affect the final reaction of the kit, and why is its removal recommended? Additionally, what is the significance of the absence of enzymes in the kit for converting glucose and lactate to pyruvate in relation to the buffer?

    1 answer

    1. The kit's final reaction is dependent on the oxidation of pyruvate, so its presence in the buffer may interfere with the assay. If possible, it is recommended to remove pyruvate from the Krebs buffer. However, the kit does not contain enzymes that can convert glucose to pyruvate or lactate to pyruvate, so the presence of these should not pose an issue in the buffer.

      Helpful?

  7. Can I use the kit for both mouse and human serum?

    1 answer

    1. This kit is suitable for mouse and human serum. As mentioned on the Product Detail Page, this item is suitable for measuring alanine aminotransferase (ALT) activity in biological samples including tissue, cells, and serum.

      Helpful?

  8. Can it be used for measuring on supernatant from liver cells

    1 answer

    1. The kit is optimized for use in determination of alanine aminotransferase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.) This kit has not been tested for use with cell culture supernatant. The presence of phenol red may interfere with results. In addition, the cultured cell line may not secrete sufficient ALT to be detected. It would be up to the end user to determine suitability.

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