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M7279

N,N′-Methylenebisacrylamide

powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

Synonyme(s) :

BIS

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A propos de cet article

Formule linéaire :
(CH2=CHCONH)2CH2
Numéro CAS:
Poids moléculaire :
154.17
UNSPSC Code:
41105319
NACRES:
NB.22
PubChem Substance ID:
EC Number:
203-750-9
Beilstein/REAXYS Number:
1706297
MDL number:
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grade

Molecular Biology

Quality Segment

assay

≥99.5%

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO4 ) (ICP)

conductivity

≤10 μmho (2% solution)

anion traces

chloride (Cl-): ≤50 mg/kg

cation traces

Ca: ≤10 ppm, Cd: ≤5 ppm, Co: ≤5 ppm, Cr: ≤5 ppm, Cu: ≤5 ppm, Fe: ≤5 ppm, K: ≤50 ppm, Mg: ≤5 ppm, Mn: ≤5 ppm, Na: ≤50 ppm, Ni: ≤5 ppm, Pb: ≤5 ppm, Zn: ≤5 ppm

absorption

≤0.2 at 290 at 1%

foreign activity

DNase and RNase, none detected

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Application

N,N′-Methylenebisacrylamide has been used:
  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
  • as a crosslinker to prepare tough hydrogel network
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

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Cet article
M7256101546M1533
description

powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

description

suitable for electrophoresis (after filtration or allowing insolubles to settle)

description

for electrophoresis, special grade for molecular biology

description

suitable for electrophoresis, 2% in H2O

form

powder

form

powder

form

crystalline powder

form

liquid

grade

Molecular Biology

grade

-

grade

-

grade

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

foreign activity

DNase and RNase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

impurities

≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO4 ) (ICP)

impurities

-

impurities

-

impurities

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

200


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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