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M7257

α-Mannosidase from Canavalia ensiformis (Jack bean)

Name: α-Mannosidase from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

Synonyme(s) :

α-D-Mannoside mannohydrolase

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A propos de cet article

Numéro CAS:

9025-42-7

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-794-1

MDL number:

MFCD00130495

Numéro CE :

3.2.1.24 (BRENDA, IUBMB)

Specific activity:

≥15 units/mg protein (biuret)

Biological source:

Canavalia ensiformis

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Propriétés

biological source

Canavalia ensiformis

form

ammonium sulfate suspension

specific activity

≥15 units/mg protein (biuret)

foreign activity

β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%

storage temp.

2-8°C

SMILES string

[Zn+2]

InChI

1S/Zn/q+2

InChI key

PTFCDOFLOPIGGS-UHFFFAOYSA-N

Description

General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.

Liberates mannose from a variety of synthetic and natural α-mannosides.

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:

for the digestion of neutral glycans.
to remove O-glycans from recombinant β-glucosidase
for the inhibition of renal fibroblasts proliferation

Biochem/physiol Actions

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c