M7133

4-Methylumbelliferyl sulfate potassium salt

sulfatase substrate

• Synonyme(s) : Potassium 4-methylumbelliferyl sulfate
• Formule empirique (notation de Hill) : C₁₀H₇KO₆S
• Numéro CAS: 15220-11-8
• Poids moléculaire : 294.32
• UNSPSC Code: 12352204
• NACRES: NA.32
• PubChem Substance ID: 24897141
• EC Number: 239-272-2
• Beilstein/REAXYS Number: 3803203
• MDL number: MFCD00016970

Propriétés

• Quality Segment: 200
• assay: ≥99% (HPLC)
• form: powder
• solubility: water: 5 mg/mL, clear, colorless
• fluorescence: λ_ex 334 nm; λ_em 370 nm (pH 10.4), λ_ex 360 nm; λ_em 449 nm (Reaction products)
• storage temp.: −20°C
• SMILES string: [K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12
• InChI: 1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1
• InChI key: CSOCSPXOODWGLJ-UHFFFAOYSA-M

Description

General description

4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).

Application

4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from 3′-phosphoadenosine 5′-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.

Biochem/physiol Actions

4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)