The MEM vitamin solution is a published classical formulation containing numerous components. See the link below to review the formulation of this and other classical vitamin and amino acid mixtures:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/167/485/m6895for.pdf
M6895
MEM Vitamin Solution (100×)
sterile-filtered, BioReagent, suitable for cell culture
Synonyme(s) :
MEM media, MEM vitamin solution
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About This Item
Code UNSPSC :
12352205
eCl@ss :
32160406
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Stérilité
sterile-filtered
Niveau de qualité
Gamme de produits
BioReagent
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Couleur
yellow to dark yellow
pH
7.0-8.0
Application(s)
agriculture
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
MEM Vitamin Solution (100×) is a sterile-filtered bioreagent, which is suitable for cell culture.
Synthetic/ basal media used currently contain amino acids, carbohydrates, vitamins and salts. Eagle′s minimum essential medium (MEM) is a modified version of BME (basal medium Eagle′s), which contains increased amounts of each amino acid.[1] Addition of water soluble vitamins such as, inositol to various culture facilitates the hatching of hamster and rabbit blastocysts. Vitamins also influence the metabolism of glucose in sheep and mouse embryos.[2]
Application
MEM Vitamin Solution (100×) has been used in:
- glial conditioned medium for primary neuronal culture[3]
- in Luria-Bertani (LB) medium or minimal medium (MM)[4]
- in private medium (PM = aminoacid-starved medium) for the Aa deprivation experiments[5]
MEM Vitamin Solution (100×) is used for the following applications:
Adding supplements of vitamins to media stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
- In the preparation of DMEM medium (for primary neural cell culture)[6][3][7]
- For the maintenance of cell lines in Eagle′s Minimum Essential Medium (the medium is supplemented with MEM Vitamin Solution along with other components)[8]
Adding supplements of vitamins to media stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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MEM ビタミン溶液（100X）の組成を教えていただきたいです。
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