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M2128

Bleu de thiazol (thiazolyl blue tetrazolium bromide)

98%, powder

Synonyme(s) :

Bromure de 3-(4,5-diméthyl-2-thiazolyl)-2,5-diphényl-2H-tétrazolium, Bromure de méthylthiazolyldiphényl-tétrazolium, MTT

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100 mg

Disponible dès aujourd'huiDeSchnelldorf

31,20 €
250 mg

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52,70 €
500 mg

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93,90 €
1 g

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147,00 €
5 g

Disponible dès aujourd'huiDeSchnelldorf

591,00 €
10 g

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946,00 €

A propos de cet article

Formule empirique (notation de Hill) :
C18H16BrN5S
Numéro CAS:
298-93-1
Poids moléculaire :
414.32
NACRES:
NA.47
PubChem Substance ID:
24896603
UNSPSC Code:
12352111
EC Number:
206-069-5
MDL number:
MFCD00011964
Beilstein/REAXYS Number:
4081397

31,20 €

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Nom du produit

Bleu de thiazol (thiazolyl blue tetrazolium bromide), 98%

Quality Segment

200

assay

98%

form

powder

technique(s)

titration: suitable

color

dark yellow

mp

195 °C (dec.) (lit.)

solubility

2-methoxyethanol: soluble 20 mg/mL, ethanol: soluble 20 mg/mL, H2O: 5 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

Application

Le bleu de thiazol (thiazolyl blue tetrazolium bromide, MTT) a été utilisé dans les analyses suivantes :
  • Test de prolifération
  • Bio-essai sur le TNF (facteur de nécrose tumorale)
Le bleu de thiazol (thiazolyl blue tetrazolium bromide, MTT) peut être utilisé pour mesurer la prolifération cellulaire. Le MTT produit une solution jaunâtre qui est convertie en formazan bleu foncé, insoluble dans l′eau, par les déshydrogénases mitochondriales des cellules vivantes. Les cristaux bleus sont solubilisés à l′aide d′isopropanol acidifié et l′intensité est mesurée par colorimétrie à 570 nm.
Le bleu de thiazol (thiazolyl blue tetrazolium bromide, MTT) peut être utilisé pour mesurer la prolifération cellulaire. Le MTT produit une solution jaunâtre qui est convertie en formazan bleu foncé, insoluble dans l′eau, par les déshydrogénases mitochondriales des cellules vivantes. Les cristaux bleus sont solubilisés à l′aide d′isopropanol acidifié et l′intensité est mesurée par colorimétrie à 570 nm. Le MTT est utilisé comme réactif histochimique/cytochimique et pour la détection du NAD. Le MTT ne permet pas de détecter les systèmes enzymatiques liés à l′ADP dans les tissus, en raison de la fixation du cation par le piège à cyanure utilisé. Le MTT est rapidement réduit en formazan, qui forme un chélate avec le nickel, le cuivre et le cobalt ; le chélate à base de cobalt a été utilisé dans des systèmes oxydatifs.

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Cet article
M5655T437588190
Bleu de thiazol (thiazolyl blue tetrazolium bromide) 98%

Sigma-Aldrich

M2128

Bleu de thiazol (thiazolyl blue tetrazolium bromide)

Bleu de thiazol (thiazolyl blue tetrazolium bromide) powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

Sigma-Aldrich

M5655

Bleu de thiazol (thiazolyl blue tetrazolium bromide)

Tetrazolium Blue Chloride used in colorimetric determination of reducing compounds

Sigma-Aldrich

T4375

Tetrazolium Blue Chloride

Tetrazolium Blue Chloride suitable for microbiology, ≥90% (T)

Sigma-Aldrich

88190

Tetrazolium Blue Chloride

description

98%

description

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

description

used in colorimetric determination of reducing compounds

description

suitable for microbiology, ≥90% (T)

technique(s)

titration: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

color

dark yellow

color

-

color

-

color

-

solubility

2-methoxyethanol: soluble 20 mg/mL, H2O: 5 mg/mL

solubility

H2O: 5 mg/mL

solubility

methanol: 50 mg/mL, clear

solubility

methanol: 0.5 g/10 mL ( hot)

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

microbiology

form

powder

form

powder

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

1–10 of 10 Questions  

  1. I used 0.05g in 10ml then syringe it and add 40ml. My question is how to calculate the percentage of viable cells? I used DMSO after MTT solution

    1 answer

    1. MTT is typically used for the semi-quantitative colorimetric measurement of cell viability. This analysis is performed in a multi-well format which screens for the viability of any given compound at any given concentration. The percentage of viable cells cannot be determined using this method. After adding a stop reagent, in this case DMSO, the formazan crystals will be dissolved, making manual counting a challenge, at best. When used in a multi-well plate format, cells are incubated, a stop solution is added, and the plate is read spectrophotometrically at 570 nM. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/305/017/m2128pis.pdf

      For additional information, see the link below to the MTT kit protocol for product TOX1, a cytotoxicity kit that utilized MTT:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      To measure cell viability as a percentage of an entire population, microscopic methods may be of interest. Reagents such as Trypan Blue allow for the clear identification of viable and non-viable cells. To review more information regarding this method, please see the link below:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/cell-quantification

      Helpful?

  2. What is the recommendation volume of 5 mg/ml of thiazolyl blue tetrazolium blue (in uL) per well to use for MTT assay? 

    1 answer

    1. Instructions for use are not provided for this product. However, there is an in vitro toxicology assay kit MTT based available. The catalog number is TOX1-1KT. In the kit, 15 mg of solid MTT is supplied. This is resuspended with 3 ml of media or a balanced salt solution without phenol red or serum. The link shared below will list the complete procedure

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      Helpful?

  3. Is the MTT assay quantitative?

    1 answer

    1. The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.

      Helpful?

  4. What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. We test the solubility of MTT in water at a concentration of 5 mg/mL.  For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.

      Helpful?

  5. How can I store solutions of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. Reconstituted MTT solution is stable for at least 6 months when stored frozen (-20 °C). Storage of reconstituted MTT solution at 2-8°C for more than 2 weeks may cause decomposition and yield erroneous results.

      Helpful?

  6. How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?

    1 answer

    1. Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source:  Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.

      Helpful?

  7. What type of multi-well plate should be used for the Thiazolyl Blue Tetrazolium Bromide (MTT) assay?

    1 answer

    1. It is recommended that flat bottom multi-well plates be used for the MTT assay. The use of tissue culture treated plates is only important if necessary for good cell growth.  If cells are grown in larger plates, the supernatant after cell lysis can be transferred to a 96 well plate for reading.

      Helpful?

  8. What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?

    1 answer

    1. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).

      Helpful?

  9. Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate.  It also corrects for dust and fingerprints that can be present.  The assay is read at 570 nm.  The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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