The LPS is in an aqueous solution containing 0.5% triethylamine and pH adjusted to 6.5 using hydrochloric acid.
L5543
Lipopolysaccharides from Escherichia coli O26:B6
Ready Made solution, 1 mg/mL, 0.2 μm filtered
Synonyme(s) :
LPS
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Laissez-nous vous aiderbiological source
Escherichia coli (O26:B6)
Quality Segment
sterility
0.2 μm filtered
form
aqueous solution
concentration
1 mg/mL
color
colorless to faint yellow
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
General description
This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O26:B6. This LPS serotype has been used in studies of septic shock.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Cet article | |||
|---|---|---|---|
| description Ready Made solution, 1 mg/mL, 0.2 μm filtered | description purified by trichloroacetic acid extraction | description γ-irradiated, BioXtra, suitable for cell culture | description ≥10,000 EU/mg, purified by phenol extraction |
| biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O26:B6) |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| solubility water: soluble | solubility water: soluble | solubility H2O: 5 mg/mL, slightly hazy | solubility water: soluble |
| form aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color colorless to faint yellow | color white to yellow | color - | color white to faint yellow |
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