L5293

Lipopolysaccharides from Escherichia coli O111:B4

Ready Made solution, 1 mg/mL

• Synonyme(s) : LPS
• Numéro CAS: 93572-42-0
• UNSPSC Code: 12352201
• NACRES: NA.25
• MDL number: MFCD00164401

Propriétés

• biological source: Escherichia coli (O111:B4)
• Quality Segment: 200
• form: aqueous solution
• concentration: 1 mg/mL
• color: colorless to faint yellow
• solubility: water: soluble
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

Lipopolysaccharides from Escherichia coli O111:B4 has been used:

• to stimulate an inflammatory response in porcine liver tissues in-vivo and piglet ileum tissues ex vivo
• to stimulate Ex vivo natural killer(NK) cells and hepatic B cells
• to induce arthritis in a standard rabbit model

Les lipopolysaccharides (LPS) sont des éléments constitutifs caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Les LPS et leur fraction lipidique (lipide A) stimulent les cellules du système immunitaire inné par l'intermédiaire du récepteur TLR4 (Toll-Like Receptor 4), qui appartient à la famille des récepteurs de type Toll et qui reconnaît la signature moléculaire des principaux agents pathogènes (PAMP).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 1
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)