This product (lipopolysaccharide from E. coli O111:B4) is classified as a smooth (S-type) lipopolysaccharide. Please see the link below to review a publication that may be helpful:
https://www.ncbi.nlm.nih.gov/books/NBK554414/
L2630
Lipopolysaccharides from Escherichia coli O111:B4
purified by phenol extraction
Synonyme(s) :
LPS
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Escherichia coli (O111:B4)
Quality Segment
form
lyophilized powder
purified by
phenol extraction
technique(s)
ELISA: suitable, cell based assay: suitable, ligand binding assay: suitable
impurities
≤3.00% protein (Lowry-TCA)
color
off-white to faint yellow
solubility
water: soluble
application(s)
cell analysis
clinical research
life science and biopharma
shipped in
ambient
storage temp.
2-8°C
General description
Un lipopolysaccharide est un glycolipide constitué de glucides liés à des acides gras. Les lipopolysaccharides (LPS) sont des constituants caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Une molécule de LPS typique contient le lipide A (phospholipide de type glucosamine), un oligosaccharide central relativement court et un polysaccharide distal (antigène O). Les LPS contribuent à l'intégrité de la membrane externe et protègent la cellule contre l'action des sels biliaires et des antibiotiques lipophiles. Ils stimulent les cellules hôtes en activant les récepteurs de type Toll (toll like receptors) TLR2 et TLR4. Cette stimulation induit la génération de diverses cytokines pro-inflammatoires, telles que TNFα (tumor necrosis factor, facteur de nécrose tumorale), IL-1 (interleukine 1) et IL-6.
Ce produit est extrait au phénol à partir d'Escherichia coli de sérotype O111:B4. La souche source provient d'une collection privée. Ce sérotype de LPS a été utilisé pour stimuler les lymphocytes B et induire l'oxyde nitrique synthase (NOS, nitric oxide synthase) dans les hépatocytes humains.
Ce produit est extrait au phénol à partir d'Escherichia coli de sérotype O111:B4. La souche source provient d'une collection privée. Ce sérotype de LPS a été utilisé pour stimuler les lymphocytes B et induire l'oxyde nitrique synthase (NOS, nitric oxide synthase) dans les hépatocytes humains.
Application
Les lipopolysaccharides (LPS) issus de Escherichia coli O111:B4 ont été utilisés :
- pour stimuler des macrophages.
- pour un préconditionnement aux LPS chez des souris.
- pour induire une lésion rénale aiguë chez des souris.
- pour induire un syndrome de détresse respiratoire aiguë (SDRA) chez des souris afin d'étudier les effets anti-inflammatoires d'exosomes dérivés de cellules souches mésenchymateuses de moelle osseuse (BMSC).
- pour induire un état hyper-inflammatoire chez des souris infectées par le coronavirus du syndrome respiratoire aigu sévère 2 (SARS-CoV-2).
Les lipopolysaccharides (LPS) sont des éléments constitutifs caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Les LPS et leur fraction lipidique (lipide A) stimulent les cellules du système immunitaire inné par l'intermédiaire du récepteur TLR4 (Toll-Like Receptor 4), qui appartient à la famille des récepteurs de type Toll et qui reconnaît la signature moléculaire des principaux agents pathogènes (PAMP).
Biochem/physiol Actions
Les lipopolysaccharides (LPS) constituent une partie de la membrane externe, ce qui rend cette dernière perméable aux molécules hydrophobes telles que les substances antimicrobiennes qui procurent une résistance innée aux bactéries à Gram négatif. Les LPS agissent sur les interactions bactérie-hôte qui régulent les réponses du système hôte.
Preparation Note
Ce produit est soluble dans l′eau (5 mg/ml) ou dans du milieu de culture cellulaire (1 mg/ml) et donne une solution trouble jaune pâle. Une solution plus concentrée (20 mg/ml), toujours trouble, a été obtenue dans une solution saline aqueuse après mélange au vortex et chauffage à 70-80 °C. Les lipopolysaccharides sont des molécules qui forment des micelles dans tous les solvants. Des solutions troubles sont observées dans l′eau et la solution tampon phosphate. Les solvants organiques ne produisent pas une solution plus limpide. Le méthanol donne une suspension turbide avec des particules flottantes, tandis que l′eau produit une solution trouble homogène.
Other Notes
Pour bien comprendre notre vaste gamme de lipopolysaccharides et tout ce qu'elle peut apporter à vos recherches, nous vous encourageons à visiter notre page dédiée aux glucides.
Pour de plus amples informations sur notre gamme de Produits biochimiques, merci de compléter ce formulaire.
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Cet article | |||
|---|---|---|---|
| description purified by phenol extraction | description purified by ion-exchange chromatography, TLR ligand tested | description purified by trichloroacetic acid extraction | description purified by gel-filtration chromatography |
| biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O111:B4) |
| technique(s) ELISA: suitable, cell based assay: suitable, ligand binding assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
Classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Eyeshields, Gloves, type N95 (US)
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Is this product considered a rough strain or smooth strain?
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Can I resuspend this LPS in PBS instead of water, without hindering its performance ?
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This product can be resuspended in PBS instead of water. Both water and PBS are suitable solvents, and a hazy solution will form in either.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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When I compared COAs of two batches of LPS (L2630) we have been using, I realized the potency of the newer one is 10-fold higher than the older one (30000000 and 3000000 EU/mg). Is it common for LPS that different lots have such different potency?
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Significant lot-to-lot variability in potency is quite normal for this product. It is a somewhat crude phenol extract; it is not chromatographically purified. That is, a chromatographically purified LPS such as Product L3012 would be expected to have better lot-to-lot consistency. Note that for both this product and L3012, the potency specification is greater than or equal to 500,000 Endotoxin units per milligram (EU/mg); there is a lower limit, but there is no upper limit.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is there any harm in filter sterilizing a solution with this LPS before using it in cell culture or animals? Meaning does it change or lose potency if it is sterile filtered say through a 0.2 micron filter?
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Sterile filtration should not affect the concentration or properties of the solution if the LPS is fully dissolved, but please keep in mind that this is a research-grade material that has not been best tested on animals. For a cell culture grade material that has already been γ-irradiated, please consider the following: https://www.sigmaaldrich.com/product/sigma/l4391
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I received LPS L2630 at Room Temperature. Is it ok to keep it at Room Temperature before reconstitution, although recommendations are to store it between 2-8 C?
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This product ships routinely at ambient temperature and is stable up to 2 weeks to allow for transit time. The item is to be stored at 2-8°C when received.
Please see the link below to review the product storage and shipping guidelines:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdfHelpful?
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Is product L2630 suitable for use in mammalian cell culture?
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Specific testing or validation for the use of product L2630 in mammalian cell culture has not been conducted.
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Hi i recieved L2630 10mg Lipopolysaccharides from Escherichia coli O111:B4 i will used in experimental work. how i will dilute it
1 answer-
The product is soluble in water (5 mg/mL) or cell culture medium (1 mg/mL). Please see the link below to review additional preparation and storage information:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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What is the purity level of this product ? is it ultrapure? 100% pure LPS ?
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This product is not tested for purity. However, it is tested for potency on a lot-specific basis.
Kindly review a sample CoA accessible at this link: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L2630-VAR0000271356.pdf
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