I6529

IMDM

With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonyme(s) : IMDM
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217469

Propriétés

• Nom du produit: Milieu de Dulbecco modifié par Iscove, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | hybridoma: suitable, cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: glucose: yes; phenol red: yes; NaHCO₃: yes; L-glutamine: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:

• human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers[1]
• to culture synovial tissue samples[2]
• bacterial strain inoculum for stimulation of immune cell experiments[3]

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable