H8791

Transforming Growth Factor-β3 human

HumanKine^®, 95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonyme(s) : TGF-β3
• Numéro MDL: MFCD00213054
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Transforming Growth Factor-β3 human, TGF-β3, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in HEK 293 cells
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: ≤1.0 ng/mL EC₅₀
• Qualité: endotoxin tested
• Poids mol.: dimer 25 kDa (non-glycosylated)
• Conditionnement: pkg of 5 μg
• Fabricant/nom de marque: HumanZyme
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Numéro d'accès UniProt: P10600
• Température de stockage: −20°C
• Informations sur le gène: human ... TGFB3(7043)

Description

Description générale

TGF-β3 (transforming growth factor-β3) belongs to the TGF β superfamily. The TGFβ3 gene is mapped to human chromosome 14q24.3

Actions biochimiques/physiologiques

TGF-β3 (transforming growth factor-β3) regulates lymphocyte proliferation, apoptosis, hematopoiesis and embryogenesis to maintain immune homeostasis. TGF-β plays an important role in cell growth, differentiation, and survival. TGF-β3 specifically promotes chondrogenic differentiation.TGF-β3 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β3 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β isoforms is known to participate in wound healing and tissue fibrosis. TGF-β3 is crucial for tissue restoration and scarless tissue repair. Mutation in the TGFβ3 gene is associated with development of non-syndromic cleft palate only (NS CPO), a rare congenital disease.

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Notes préparatoires

HumanKine TGF-β3 is expressed in human HEK 293 cells as a mature, disulfide linked, non- glycosylated homodimer with a predicted molecular mass of 25 kDa.
This product is lyophilized from a solution of 50mM NaOAc, pH 3.7.

Remarque sur l'analyse

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable