If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
H0887
HEPES solution
1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent
Synonyme(s) :
Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)
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A propos de cet article
Nom du produit
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
sterility
sterile-filtered
Quality Segment
product line
BioReagent, BioXtra
assay
0.9—1.1 M
form
liquid
storage condition
(Tightly closed. Dry), dry at room temperature
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
colorless
pH
7.0-7.6
useful pH range
6.8-8.2
7.5
density
1.1 g/cm3
suitability
suitable for component for culture media, suitable for electrophoresis
application(s)
advanced drug delivery
cell analysis
life science and biopharma
sample preparation
storage temp.
2-8°C
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
Il surpasse également les tampons au bicarbonate (NaHCO3) en termes de capacité à maintenir un pH physiologique malgré les fluctuations de concentration en dioxyde de carbone liées à la respiration des cellules, un facteur crucial pour le maintien de l'homéostasie cellulaire. Outre ses performances exceptionnelles en culture cellulaire, l'HEPES peut aussi être utilisé dans diverses études biologiques et biochimiques. Il peut aussi présenter un intérêt pour certaines techniques comme l'immunoprécipitation, la lyse cellulaire et l'imagerie de cellules vivantes, en offrant aux méthodes expérimentales un environnement stable et maîtrisé. Sa polyvalence, sa capacité de tamponnage du pH exceptionnelle et ses interactions minimales avec les autres molécules font de l'HEPES un outil indispensable dans différents domaines de recherche.
Application
- de la solution saline équilibrée de Hank, du milieu de Eagle modifié par Dulbecco et du milieu DMEM sans glucose, qui servent à la préparation de coupes tissulaires.
- de tampons utilisés dans des mesures de conductance localisées sur le côté basolatéral d'inserts de culture pour étudier la sélectivité cationique.
- de milieux de culture cellulaire pour leur conférer un pouvoir tampon permettant de maintenir le pH de lignées cellulaires.
- d'un tampon de décellularisation de nucleus pulposus de porc.
Features and Benefits
- Produit de grande pureté pour la recherche en biochimie et en biologie.
- Stérilisé par filtration et testé pour la culture cellulaire.
- Solution prête à l'emploi qui réduit le temps de préparation.
- Produit avec un pKa de 7,5 à 25 °C, à utiliser dans la plage de pH de 6,8 à 8,2
Preparation Note
Other Notes
Disclaimer
1 of 1
Cet article | |||
|---|---|---|---|
| description 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture | description BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered | description BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered | description BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration) |
| assay 0.9—1.1 M | assay - | assay - | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| concentration 1 M | concentration 1 M | concentration 1 M | concentration - |
| product line BioReagent, BioXtra | product line - | product line - | product line BioXtra |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| form liquid | form liquid | form liquid | form powder |
| assay 0.9—1.1 M | assay - | assay - | assay ≥99.5% (titration) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| concentration 1 M | concentration 1 M | concentration 1 M | concentration - |
| product line BioReagent, BioXtra | product line - | product line - | product line BioXtra |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 400 |
| form liquid | form liquid | form liquid | form powder |
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Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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Hello! Is it dissolved in H2O entirely? or does it have other surfactants?
1 answer-
This product is comprised of HEPES free acid in tissue culture grade water. No other additives or preservatives are present, including surfactants.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Bonjour, Est-ce que ce produit H0887 peut-être congelé à -80°C sans risque de le faire précipiter ?
1 answer-
It is best not to freeze the product as received. However, should the product be inadvertently frozen, it is best to thaw the product slowly. Do not place the frozen product in an incubator or water bath. Once thawed, inspect for any precipitates before use. A clear, colorless solution will be suitable for further use. Should this buffer be required to prepare the stock solution of another reagent that requires freezer storage, thaw slowly if possible.
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