H0135

Hydrocortisone

γ-irradiated, powder, BioXtra, suitable for cell culture

• Synonyme(s) : 11β,17α,21-trihydroxypregn-4-ène-3,20-dione, 4-pregnène-11β,17α,21-triol-3,20-dione, Composé F de Kendall, Substance M de Reichstein
• Formule empirique (notation de Hill): C₂₁H₃₀O₅
• Numéro CAS: 50-23-7
• Poids moléculaire : 362.46
• Numéro Beilstein : 1354819
• Numéro CE : 200-020-1
• Numéro MDL: MFCD00011654
• Code UNSPSC : 12352209
• ID de substance PubChem : 24895394
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: synthetic (organic)
• Niveau de qualité: 200
• Stérilité: γ-irradiated
• Gamme de produits: BioXtra
• Pureté: ≥80% (HPLC)
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• Pf: 211-214 °C (lit.)
• Solubilité: ethanol: 1 mg/mL, clear
• Conditions d'expédition: ambient
• Température de stockage: room temp
• Chaîne SMILES: [H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
• InChI: 1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
• Clé InChI: JYGXADMDTFJGBT-VWUMJDOOSA-N
• Informations sur le gène: human ... ABCB1(5243) , CYP3A4(1576) , NR3C1(2908) , SERPINA6(866) ; mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126) ; rat ... Ar(24208) , Nr3c1(24413)

Description

Description générale

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Application

Hydrocortisone is used for the following applications:

• Used in myogenic induction and identification
• Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
• Used in adipogenic differentiation (one of the components in adipogenic medium)
• Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
• The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
• Used in differentiation assays of melanoma spheroid cells

Actions biochimiques/physiologiques

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na²⁺ retention potency.

Forme physique

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H360Df,H373
• Conseils de prudence: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Repr. 1A - STOT RE 2
• Organes cibles: Adrenal gland,Bone marrow
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges