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GE28-9042-67

illustra™ Plasmid Midi (25)

Cytiva 28-9042-67, pack of 1 × 25 pieces

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52

Conditionnement

pack of 1 × 25 pieces

Fabricant/nom de marque

Cytiva 28-9042-67

Description générale

PlasmidPrep Midi Flow Kit is a gravity flow plasmid prep kit designed for the purification of large yields of transfection-grade plasmid DNA that is proportional to the input sample volume.

The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.

PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.

PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.

Caractéristiques et avantages

  • Provides plasmid DNA with excellent purity, > 70% (strain-dependent) of supercoiled plasmid DNA
  • Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
  • Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.
  • Consistently produces pure plasmid DNA with low levels of endotoxin contamination, < 10 endotoxin units (EU) per μg of plasmid DNA

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

illustra is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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Articles

General Considerations for Plasmid DNA Preparation

This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.

Plasmid DNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.

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