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G9145

[Leu15]-gastrine I humaine

≥95% (HPLC)

Synonyme(s) :

[Leu15]-HG-17, Heptadécapeptide [leucine15]-gastrine I humain, Heptadécapeptide [leucine15]-gastrine I, LHG-17, [Acide 18-pyroglutamique, 32-leucine]-grande gastrine I-(18-34)-peptide amide, humain, [Leu15]-gastrine I humaine, [Leucine15]-petite gastrine I

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A propos de cet article

Formule empirique (notation de Hill) :
C98H126N20O31
Numéro CAS:
39024-57-2
Poids moléculaire :
2080.16
UNSPSC Code:
12352209
NACRES:
NA.32
PubChem Substance ID:
24895364
EC Number:
254-250-2
Beilstein/REAXYS Number:
5227423
MDL number:
MFCD00076502
Form:
powder
Assay:
≥95% (HPLC)
Biological source:
human
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biological source

human

Quality Segment

assay

≥95% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

−20°C

SMILES string

CC(C)CC(NC(=O)C(Cc1c[nH]c2ccccc12)NC(=O)C3CCCN3C(=O)CNC(=O)C4CCC(=O)N4)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(C)C(=O)NC(Cc5ccc(O)cc5)C(=O)NCC(=O)NC(Cc6c[nH]c7ccccc67)C(=O)NC(CC(C)C)C(=O)NC(CC(O)=O)C(=O)NC(Cc8ccccc8)C(N)=O

InChI

1S/C98H126N20O31/c1-49(2)38-68(115-96(147)72(43-55-46-101-60-19-12-10-17-58(55)60)117-98(149)74-20-13-37-118(74)77(122)48-103-86(137)61-25-31-75(120)105-61)93(144)111-66(30-36-82(131)132)92(143)110-65(29-35-81(129)130)91(142)109-64(28-34-80(127)128)90(141)108-63(27-33-79(125)126)89(140)107-62(26-32-78(123)124)88(139)104-51(5)85(136)113-70(41-53-21-23-56(119)24-22-53)87(138)102-47-76(121)106-71(42-54-45-100-59-18-11-9-16-57(54)59)95(146)114-69(39-50(3)4)94(145)116-73(44-83(133)134)97(148)112-67(84(99)135)40-52-14-7-6-8-15-52/h6-12,14-19,21-24,45-46,49-51,61-74,100-101,119H,13,20,25-44,47-48H2,1-5H3,(H2,99,135)(H,102,138)(H,103,137)(H,104,139)(H,105,120)(H,106,121)(H,107,140)(H,108,141)(H,109,142)(H,110,143)(H,111,144)(H,112,148)(H,113,136)(H,114,146)(H,115,147)(H,116,145)(H,117,149)(H,123,124)(H,125,126)(H,127,128)(H,129,130)(H,131,132)(H,133,134)

InChI key

CMVMLPDUAGUTOC-UHFFFAOYSA-N

Gene Information

human ... GAST(2520)

General description

Le gène GAST code pour la protéine [Leu15]-gastrine I chez l′homme. Le gène GAST est situé dans la région chromosomique 17q21.2 de l′homme. Les cellules G de l′antre de l′estomac produisent le précurseur de la [Leu15]-gastrine I, la progastrine. Cette dernière subit un clivage et un traitement pour donner la gastrine, qui a un rôle trophique pour l′ensemble de l′épithélium gastro-intestinal. La gastrine est essentielle à la croissance du système digestif et stimule la production d′acide gastrique par les cellules pariétales. La gastrine exerce sa fonction via le récepteur couplé aux protéines G appelé récepteur de la cholécystokinine B (CCK), ou récepteur CCK-B (CCK-BR). La libération de gastrine est stimulée par l′alimentation, en particulier les régimes protéinés, et est inhibée par les faibles pH. La gastrite atrophique, une infection à Helicobacter pylori, et l′administration sur le long terme d′inhibiteurs de la pompe à protons, peuvent provoquer une surexpression de la gastrine. L′adénocarcinome gastrique présente de hauts niveaux de gastrine.

Application

La gastrine a été utilisée :
  • Comme supplément pour un milieu destiné à des cultures entéroïdes.
  • Comme supplément pour un milieu de Eagle modifié par Dulbecco (DMEM) standard, un milieu « minigut » humain destiné à la croissance de cryptes.

Biochem/physiol Actions

Analogue de la gastrine I possédant la totalité de son activité biologique et présentant une stabilité accrue en solution aqueuse. Leu15 remplace Met15, qui est rapidement oxydée et provoque une perte d′activité biologique.

Other Notes

Produit lyophilisé à partir d′une solution de TFA à 0,1 % dans l′eau

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Cet article
A9650324831I3769
[Leu15]-gastrine I humaine ≥95% (HPLC)

Sigma-Aldrich

G9145

[Leu15]-gastrine I humaine

Angiotensin I human acetate salt hydrate ≥90% (HPLC), powder, suitable for mammalian cell culture

Sigma-Aldrich

A9650

Angiotensin I human acetate salt hydrate

EGF, Human, Recombinant, E. coli

Sigma-Aldrich

324831

EGF, Human, Recombinant, E. coli

Insulin-like Growth Factor-I human IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

I3769

Insulin-like Growth Factor-I human

description

≥95% (HPLC)

description

≥90% (HPLC), powder, suitable for mammalian cell culture

description

-

description

IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

biological source

human

biological source

-

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

assay

≥95% (HPLC)

assay

≥90% (HPLC)

assay

≥95% (SDS-PAGE)

assay

≥95% (HPLC)

form

powder

form

powder

form

solid

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Gene Information

human ... GAST(2520)

Gene Information

human ... AGT(183)

Gene Information

-

Gene Information

human ... IGF1(3479), Igf1(16000)

Classe de stockage

11 - Combustible Solids

Que se passe-t-il ?

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

EPI (équipement de protection individuelle)

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number

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Questions

1–7 of 7 Questions  

  1. Could you please provide information regarding the storage duration?

    1 answer

    1. This product is assigned a retest date of 5 years from the date of quality release. A firm expiration date is not assigned. The retest date is lot specific and available in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/g9145#product-documentation

      The solution stability of this material has not been investigated. In general, peptide solutions may be stable up to 1 week when stored at 2-8°C. Should long term storage be necessary, storing in aliquots at -20°C is suggested. Avoid repeated freeze/thaw cycles. Note that peptides have a tendency to stick to plastic containers upon storage.

      Helpful?

  2. Can you reconstitute the powder directly into 0.1 % BSA/PBS?

    1 answer

    1. This product is tested for solubility at 1 mg/ml in 0.1% NaOH or 1% NH4OH. Other solvents have not been tested in-house. This product was lyophilized from 0.1% TFA, which is a strong acid. Reconstituting in 0.1% NaOH would help to neutralize the acid. Reconstituting in PBS will not damage the gastrin, but the solution may still be acidic. There may be instances where the gastrin is more difficult to resuspend in PBS.

      Helpful?

  3. Is the powder sterile for cell culture use

    1 answer

    1. This item is not a sterile product. Stock solutions may be filter sterilized.

      Helpful?

  4. After preparing aliquots, what is the stability of this product at -20?

    1 answer

    1. The stability of this product in solution has not been evaluated. Generally, peptides should not be stored in solution for extended periods. Should solution storage be necessary, storing aliquots at -20°C is suggested. Note that peptides have a tendency to stick to plastic containers upon storage.

      Helpful?

  5. Is this product also suitable for use with other mammalian species, such as mouse, for ex vivo tissue studies, as opposed to just human organoid culture medium?

    1 answer

    1. The product's validation for batch release does not currently cover murine ex vivo tissue testing. However, the following citations may provide insights into its application for ex vivo non-human samples:

      Sci Adv. 2023 Nov 17;9(46):eadh0322. Title: Inactivation of the tumor suppressor gene Apc synergizes with H. pylori to induce DNA damage in murine gastric stem and progenitor cells PMID: 37967175

      Infect Immun. 2016 Sep 19;84(10):3083-91. Title: Intestinal Enteroids Model Guanylate Cyclase C-Dependent Secretion Induced by Heat-Stable Enterotoxins PMID: 27481254

      Helpful?

  6. I am interested in this product. A colleague uses it for human organoid culture medium. However, I work with murine ex vivo tissues. Is this also applicable for other mammalian species (so mouse in stead of human)?

    1 answer

    1. This product has not been tested for use with murine ex vivo tissue. However, the following citations may be of interest for its application in ex vivo non-human samples.

      Sci Adv. 2023 Nov 17;9(46):eadh0322.
      Inactivation of the tumor suppressor gene Apc synergizes with H. pylori to induce DNA damage in murine gastric stem and progenitor cells
      PMID: 37967175
      https://pubmed.ncbi.nlm.nih.gov/37967175/

      Infect Immun. 2016 Sep 19;84(10):3083-91.
      Intestinal Enteroids Model Guanylate Cyclase C-Dependent Secretion Induced by Heat-Stable Enterotoxins
      PMID: 27481254
      https://pubmed.ncbi.nlm.nih.gov/27481254/

      Helpful?

  7. この製品の溶媒は何を用いればよろしいでしょうか。水またはPBS(-)には溶けますでしょうか、あるいはDMSOでしょうか。

    1 answer

    1. This product is tested for solubility at 1mg/ml in 0.1% NaOH or 1% NH4OH. Other solvents have not been evaluated.

      Helpful?

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