The stability of this product in solution has not been determined beyond the 1 - 2 month recommended for freezer storage. However, similar product 346774, under the CalBiochem tradename, is stable at 2 - 8°C up to 6 months when prepared at 1 mg/mL in 5 mM glycine buffer, pH 8. See the link below to review this information:
https://www.emdmillipore.com/product/Glucose-6-phosphate-Dehydrogenase-Leuconostoc-mesenteroides,EMD_BIO-346774#anchor_PDS
G8529
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)
Synonyme(s) :
G-6-P-DH
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About This Item
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Forme
lyophilized powder
Activité spécifique
≥550 units/mg protein (biuret)
Composition
Protein, 10-40% biuret
Application(s)
agriculture
Activité étrangère
creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
Température de stockage
2-8°C
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Application
Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:
- in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
- to study the starch time course using Nicotiana tabacum leaves
- to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
- to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings
Actions biochimiques/physiologiques
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.
Définition de l'unité
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
Forme physique
Lyophilized powder containing Ficoll and Tris buffer salts
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Gloves
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Les clients ont également consulté
Alyssa L Preiser et al.
Frontiers in plant science, 11, 580726-580726 (2020-12-29)
Phosphoglucoisomerase (PGI) isomerizes fructose 6-phosphate (F6P) and glucose 6-phosphate (G6P) in starch and sucrose biosynthesis. Both plastidic and cytosolic isoforms are found in plant leaves. Using recombinant enzymes and isolated chloroplasts, we have characterized the plastidic and cytosolic isoforms of
Benjamin J Reisman et al.
Nature chemical biology, 18(4), 360-367 (2021-12-04)
Cancer cells have long been recognized to exhibit unique bioenergetic requirements. The apoptolidin family of glycomacrolides are distinguished by their selective cytotoxicity towards oncogene-transformed cells, yet their molecular mechanism remains uncertain. We used photoaffinity analogs of the apoptolidins to identify
Thomas Beneyton et al.
Nature communications, 9(1), 2391-2391 (2018-06-21)
Self-sustained metabolic pathways in microcompartments are the corner-stone for living systems. From a technological viewpoint, such pathways are a mandatory prerequisite for the reliable design of artificial cells functioning out-of-equilibrium. Here we develop a microfluidic platform for the miniaturization and
Myriam Ferrara et al.
Extremophiles : life under extreme conditions, 17(1), 53-62 (2012-11-03)
Psychrophilic organisms have evolved a number of modifications of cellular structures to survive in the cold environment; among them it is worth noting an increased efficiency of enzymes at lower temperatures. Glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) was purified and characterised
Shinichi Harada et al.
The Journal of pharmacology and experimental therapeutics, 344(1), 276-285 (2012-11-03)
Orexin-A (a glucose-sensing neuropeptide in the hypothalamus) and brain-derived neurotrophic factor (BDNF; a member of the neurotrophin family) play roles in many physiologic functions, including regulation of glucose metabolism. We previously showed that the development of postischemic glucose intolerance is
Protocoles
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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If Product No. G8529, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, dissolved in Glycine 5mM, Can enzyme with Glycine buffer be stable for more than 2 months at -20°C?
1 answer-
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Can solutions of Product No. G8529, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, be stored?
1 answer-
This product may be stored in aliquots at -20°C in citrate buffer for approximately 1-2 months. Note that product G8404 is an ammonium sulfate suspension that may be stored at 2-8°C.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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In which solvent can Product No. G8529, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?
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The product can be dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, (with or without 0.1% BSA) or in deionized water at 1 mg/ml (buffer is preferable)
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What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?
1 answer-
Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli. Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.
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For Product No. G8529, Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, can the Ficoll present in the product be removed?
1 answer-
Ficoll is added to the enzyme preparation as a stabilizer. The enzyme may become unstable if the Ficoll is removed (i.e., through a dialysis step).
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What is the molecular weight of Product No. G8529, Glucose-6-phosphate Dehydrogenase?
1 answer-
Sigma has not determined the molecular weight of this protein, but a value of 55,000 daltons has been reported for the monomer molecular weight (the enzyme from Leuconostoc mesenteroides is reportedly a dimer protein) (Biochem. Biophys. Res. Comm. 59, 894 (1974)).
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