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G7877

Sigma-Aldrich

Glucose-6-phosphate déshydrogénase from baker′s yeast (S. cerevisiae)

Type VII, ammonium sulfate suspension, ≥200 units/mg protein

Synonyme(s) :

G-6-P-DH, Zwischenferment

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About This Item

Numéro CAS:
9001-40-5
Numéro de classification (Commission des enzymes):
1.1.1.49 (BRENDA, IUBMB)
Numéro CE :
232-602-6
Numéro MDL:
MFCD00081656
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bakers yeast

Niveau de qualité

200

Type

Type VII

Forme

ammonium sulfate suspension

Activité spécifique

≥200 units/mg protein

Poids mol.

128 kDa

Technique(s)

cell based assay: suitable

Solubilité

water: soluble

Application(s)

diagnostic assay manufacturing

Température de stockage

2-8°C

Informations sur le gène

Saccharomyces cerevisiae ... ZWF1(855480)

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Catégories apparentées

Description générale

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Actions biochimiques/physiologiques

La glucose-6-phosphate déshydrogénase catalyse la transformation du glucose-6-phosphate en 6-phosphogluconolacétone, ce qui constitue la première étape de la voie des pentoses phosphates.

Définition de l'unité

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Forme physique

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 7.0

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Samantha R Soncini et al.
PLoS genetics, 16(7), e1008484-e1008484 (2020-07-17)
Yeast and fast-growing human tumor cells share metabolic similarities in that both cells use fermentation of glucose for energy and both are highly sensitive to the glucose analog 2-deoxyglucose. Spontaneous mutations in S. cerevisiae that conferred resistance to 2-deoxyglucose were
Chapter 18 - Pharmacodynamic Pharmacogenomics
McCallum L, et al.
Handbook of Pharmacogenomics and Stratified Medicine, 8(12), 365-383 (2014)
Alcohol and gastrointestinal tract function
Bhardwaj SB
Bioactive Food as Dietary Interventions for Arthritis and Related Inflammatory Diseases, 81-81 (2012)
Gang Zhao et al.
Neurochemistry international, 61(2), 146-155 (2012-05-15)
NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD
Xuehuai Shen et al.
General and comparative endocrinology, 177(1), 177-186 (2012-04-03)
Dehydroepiandrosterone (DHEA), a major steroid hormone, decreases with age, and this reduction has been shown to be associated with physical health. In the present study, the effect of DHEA on cell growth and mitochondrial function was investigated using TM-3 cells
Afficher tous les articles scientifiques apparentés

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocoles

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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