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technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 12-15 mL
bead size
20-50 μm
application(s)
life science and biopharma
compatibility
Cytiva
General description
Sephadex® G-75 is well-established gel filtration medium for desalting and buffer exchange of large biomolecules >80,000 molecular weight.
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-75 is for larger molecules.
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-75 is for larger molecules.
Application
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. It has been used for desaltation process to study the production, purification, and immobilization of l-asparaginase II (ASNase II) in chitosan nanoparticles (CSNPs).
Legal Information
Sephadex is a registered trademark of Cytiva
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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