This product is produced through bacterial fermentation. The process is generally considered to be of organic origin in the biochemical sense, as it is produced by living organisms (bacteria) rather than through purely chemical synthesis.
G7513
L-glutamine solution
200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
Synonyme(s) :
Glavamin, Levoglutamide
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A propos de cet article
Numéro CAS:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
EC Number:
200-292-1
NACRES:
NA.75
eCl@ss:
32160406
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Laissez-nous vous aidersterility
sterile-filtered
Quality Segment
product line
BioXtra
form
solution
concentration
200 mM
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Gene Information
rat ... Ggt1(116568)
General description
La L-glutamine est un acide aminé essentiel qui sert d'importante source d'énergie aux cellules en culture et constitue un composant crucial des milieux de culture. La L-glutamine est très stable sous forme de poudre sèche ou de solution congelée. Elle se dégrade toutefois assez rapidement dans les milieux liquides et les solutions mères. L'atteinte de performances cellulaires optimales nécessite généralement l'ajout, au milieu, d'un supplément en L-glutamine avant utilisation.
Application
La L-glutamine est un acide aminé essentiel et un élément crucial des milieux de culture, où il constitue une source d'énergie importante pour les cellules. Pour atteindre des performances cellulaires optimales, il est généralement nécessaire de supplémenter le milieu en L-glutamine avant utilisation.
Preparation Note
Produit préparé dans de l'eau de qualité culture cellulaire.
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Cet article | |||
|---|---|---|---|
| description 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture | description ReagentPlus®, ≥99% (HPLC) | description meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source | description phyproof® Reference Substance |
| form solution | form powder | form powder | form crystals |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| product line BioXtra | product line ReagentPlus® | product line - | product line phyproof® Reference Substance |
| storage temp. −20°C | storage temp. room temp | storage temp. room temp | storage temp. - |
| impurities endotoxin, tested | impurities ≤0.2% free ammonia., ~0.5% Glutamic acid | impurities endotoxin, residual solvents, tested | impurities - |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level - |
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Classe de stockage
12 - Non Combustible Liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Guten Tag, ist das L-Glutamin biologischen Ursprungs? Danke und VG!
1 answer-
Helpful?
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How much of Product G7513 should be used in cell culture media?
1 answer-
In general, MEM typically contains 0.292 g/L L-Glutamine. A 200mM Glutamine solution is equivalent to 29.23 mg/mL. So it needs to be diluted around 100 times.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
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