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G4252

Sigma-Aldrich

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Synonyme(s) :

S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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About This Item

Numéro CAS:
9033-12-9
Numéro de classification (Commission des enzymes):
4.4.1.5 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131225
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Type

Grade IV

Forme

buffered aqueous glycerol solution

Activité spécifique

≥400 units/mg protein

Activité étrangère

glyoxalase II ≤1%

Température de stockage

2-8°C

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Description générale

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Actions biochimiques/physiologiques

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Définition de l'unité

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

Forme physique

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

Remarque sur l'analyse

Protein determined by biuret.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Xuming Jia et al.
PloS one, 7(5), e36610-e36610 (2012-05-19)
Methylglyoxal (MG) is a highly reactive metabolite physiologically presented in all biological systems. The effects of MG on diabetes and hypertension have been long recognized. In the present study, we investigated the potential role of MG in obesity, one of
Megan Jack et al.
Translational research : the journal of laboratory and clinical medicine, 159(5), 355-365 (2012-04-17)
Diabetic neuropathy is the most common and debilitating complication of diabetes mellitus with more than half of all patients developing altered sensation as a result of damage to peripheral sensory neurons. Hyperglycemia results in altered nerve conduction velocities, loss of
Víctor Cubillos et al.
Marine environmental research, 119, 59-71 (2016-05-28)
The estuarine slipper limpet Crepipatella dilatata is a gastropod that can survive prolonged periods of low salinities (< 24 PSU) caused by tidal changes and/or prolonged periods of rain. During low salinity events, C. dilatata can isolate its body from the outside
Unicameral bone cyst in the calcaneus with pathologic fracture. A literature review and case report.
N A Grumbine et al.
Journal of the American Podiatric Medical Association, 76(2), 96-99 (1986-02-01)
On glyoxalase
H.D. Dakin &amp; H.W. Dudley
The Journal of Biological Chemistry, 14, 423-431 (1913)
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