F6625

Flavin adenine dinucleotide disodium salt hydrate

≥95% (HPLC), powder

• Synonyme(s) : FAD, FAD-Na2, Riboflavin 5′-adenosine diphosphate disodium salt, FAD-Na₂
• Formule empirique (notation de Hill) : C₂₇H₃₁N₉Na₂O₁₅P₂ · xH₂O
• Numéro CAS: 84366-81-4
• Poids moléculaire : 829.51 (anhydrous basis)
• UNSPSC Code: 41106305
• NACRES: NA.51
• PubChem Substance ID: 24894909
• MDL number: MFCD08277021
• Beilstein/REAXYS Number: 5326842
• Form: powder
• Assay: ≥95% (HPLC)
• Solubility: H₂O: soluble-50 mg/mL, clear, slightly orang to deep orange
• Biological source: microbial
• Color: yellow to orange-brown

Propriétés

• biological source: microbial
• Quality Segment: 200
• assay: ≥95% (HPLC)
• form: powder
• mol wt: 829.51 g/mol
• color: yellow to orange-brown
• solubility: H₂O: soluble-50 mg/mL, clear, slightly orang to deep orange
• λmax: 375-377 nm, 448-450 nm
• fluorescence: (EmM Anhydrous 10.5 - 11.8 pH 7.0, Lambda Max 448 to 450 nm; EmM Anhydrous 8.5 - 9.5 pH 7.0, Lambda Max 375 to 377 nm)
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[H]O[H].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP([O-])(=O)OP([O-])(=O)OC[C@H]4O[C@H]([C@H](O)[C@@H]4O)n5cnc6c(N)ncnc56)c2cc1C
• InChI: 1S/C27H33N9O15P2.2Na.H2O/c1-10-3-12-13(4-11(10)2)35(24-18(32-12)25(42)34-27(43)33-24)5-14(37)19(39)15(38)6-48-52(44,45)51-53(46,47)49-7-16-20(40)21(41)26(50-16)36-9-31-17-22(28)29-8-30-23(17)36;;;/h3-4,8-9,14-16,19-21,26,37-41H,5-7H2,1-2H3,(H,44,45)(H,46,47)(H2,28,29,30)(H,34,42,43);;;1H2/q;2*+1;/p-2/t14-,15+,16+,19-,20+,21+,26+;;;/m0.../s1
• InChI key: GXTPHHZYFMAGLX-UJXBNFGUSA-L

Description

General description

Flavin adenine dinucleotide disodium salt hydrate (FAD-Na2) is an adenine-containing enzymatic redox cofactor. Also known as flavin cofactors, FAD is critical electron transporter in living systems. They catalyze several 1-2 electron redox reactions. e.g., β-oxidation of fatty acids occurs in the presence of FAD as a cofactor. FAD is one of the two active coenzymes of vitamin B12(riboflavin). FAD displays a significantly shorter excited state lifetime in aqueous solutions than its analog, flavin mononucleotide.

Application

FAD is used to remove reactive oxygen species (ROS) from mammalian cells. The fluorescence mechanism of FAD is used to study energy-dependent intramitochondrial redox potential. FAD is used as a predominant fluorophore to study unstained eosinophils, which exhibit autofluorescence compared to other leucocytes.

Flavin adenine dinucleotide (FAD) is used as a redox cofactor (electron carrier) by flavoproteins including succinate dehydrogenase (complex), α-ketoglutarate dehydrogenase, apoptosis-inducing factor 2 (AIF-M2, AMID), folate/FAD-dependent tRNA methyltransferases, and N-hydroxylating flavoprotein monooxygenases. FAD is a component of the pyruvate dehydrogenase complex.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, type N95 (US)