F3022
Anticorps monoclonal anti-β-actine
clone AC-15, purified from hybridoma cell culture
Synonyme(s) :
Monoclonal Anti-β-Actin
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
FITC conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
AC-15, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen 42 kDa
Espèces réactives
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
Ne doit pas réagir avec
Dictyostelium discoideum
Technique(s)
direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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Description générale
Dans la coloration de cryosections ultra-minces de gésiers de poulet, l′anticorps marque les corps denses et les faisceaux longitudinaux reliant les corps denses successifs, comportant également la desmine et la plaque dense associée à la membrane. Il ne marque pas les muscles cardiaques et squelettiques adultes, sauf en cas de contamination de l′échantillon par des cellules non musculaires ou lorsque du tissu embryonnaire est utilisé.
Monoclonal Anti-b-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ACTB (actin beta) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. Actin is a eukaryotic protein. It belongs to the major cytoskeletal protein family.
Immunogène
Peptide N-terminal de β-actine cytoplasmique légèrement modifié, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugué à la KLH.
Application
L′anticorps monoclonal anti-actine de souris a été utilisé comme contrôle de charge pour l′analyse par western blot de lysats de protéines du foie.
L′anticorps monoclonal anti-actine de souris a été utilisé comme contrôle de charge pour l′analyse par western blot de protéines immunoprécipitées à partir de co-cultures de ganglion spinal de rat.
Anti-β-Actin-FITC antibody, Mouse monoclonal has been used in:
- western blotting
- immunohistochemistry
- flow cytometry
- direct immunofluorescent staining
- dual immuno-fluorescent staining
Actions biochimiques/physiologiques
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.
Actins are majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Autres remarques
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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